Data Release: High-Resolution Imaging and Segmentation of P7 Mouse Tissue Microarchitecture Using FIB-SEM and Machine Learning

David Ackerman; Emma Avetissian; Christopher K. E. Bleck; John A. Bogovic; Michael Innerberger; Wyatt L. Korff; Wei-Ping Li; Zhiyuan Lu; Alyson Petruncio; Stephan Preibisch; Wei Qiu; Jeff L. Rhoades; Stephan Saalfeld; Malan Silva; Eric T. Trautman; Rebecca Vorimo; Aubrey V. Weigel; Zhiheng Yu; Yurii Zubov; CellMap Project Team

doi:10.1101/2024.09.05.611438

Cell BiologybioRxivSep 5, 2024

Preprint

Data Release: High-Resolution Imaging and Segmentation of P7 Mouse Tissue Microarchitecture Using FIB-SEM and Machine Learning

David Ackerman iD, Emma Avetissian iD, Christopher K. E. Bleck iD, John A. Bogovic iD, Michael Innerberger iD, Wyatt L. Korff iD, Wei-Ping Li iD, Zhiyuan Lu iD, Alyson Petruncio iD, Stephan Preibisch iD, Wei Qiu iD, Jeff L. Rhoades iD, Stephan Saalfeld iD, Malan Silva iD, Eric T. Trautman iD, Rebecca Vorimo iD, Aubrey V. Weigel iD, Zhiheng Yu iD, Yurii Zubov iD, CellMap Project Team

Abstract

This report presents a comprehensive data release exploring the tissue microarchitecture of P7 aged mice using Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) combined with machine learning-based segmentations of nuclei. The study includes high-resolution 3D volumes and nucleus segmentations for seven vital tissues--pancreas, liver, kidney, heart, thymus, hippocampus, and skin--from a single mouse. The detailed datasets are openly accessible onOpenOrganelle.org, providing a valuable resource for the scientific community to support further research and collaboration.

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