microscopy & imaging analysis

This study shows HMGB1 counteracts protamine to maintain liquid DNA condensates, facilitating DNA repair by preventing tangle formation.

This study uses a live-cell autophagy reporter to show that naked mole-rat skin fibroblasts form reversible vacuoles under lysosomal stress, indicating dynamic lysosomal remodelling.

The paper compares image quality and resolution between scanning electron microscopy and super-resolution structured illumination microscopy on a mammalian testis sample.

This study uses cryo-EM and charge detection mass spectrometry to determine the stoichiometry and structure of Bpa-substrate complexes in Mtb, revealing an undecameric apo form and a Bpa12:HspR2 complex.

This study shows that coiled-coil kinking in laminin-332 directs elastase cleavage to a specific hinge site, ensuring proper fragment generation, while extended conformations lead to random cleavage and degradation.

This paper introduces enhanced 2D-SIM to reduce reconstruction artifacts and improve resolution in thick samples using optimized coarse and fine patterns.

This paper introduces high-speed atomic force-Raman microscopy (HS-AFRM) to combine structural and chemical analysis, enhancing the understanding of biomolecular dynamics.

This study uses single-molecule FRET to show that gp41-directed antibodies stabilize or open the prefusion-closed state of HIV-1 Env, revealing two neutralization mechanisms.

This study identifies a chain-feeding mechanism in BRCA1-A that explains its preference for long K63-linked ubiquitin chains through avidity and processivity.

This study shows lipid nanoparticles form protein coronas through lipoprotein fusion, not surface adsorption, using cryo-EM and simulations.

The paper identifies spatially organized tertiary lymphoid structures in small cell lung cancer and links their presence to improved patient survival.

This study uses 3D FIB-SEM to analyze chromosome morphology across eight dinoflagellate species, revealing shared features and species-specific differences.

The paper links SAC silencing with mitotic memory through microtubule occupancy at kinetochores, showing that high occupancy enables timely silencing and prevents mitotic stopwatch-induced proliferation block.

The paper develops a time-resolved phenotyping workflow to continuously track rice seed germination dynamics in shallow-water culture, revealing heterogeneity in germination timing and post-emergence growth rates.

This paper determines the cryo-EM structure of the PI3KC3-C2 complex with Rubicon's PIKBD domain, revealing how Rubicon selectively inhibits PI3KC3-C2 to regulate endolysosome maturation and mitophagy.

This study investigates the role of phosphate and carbonate in the biomineralization of bivalve shells, identifying phosphate as the primary phase and carbonate as a secondary phase in Limnoperna fortunei.

This paper introduces sDNA-PAINT, a method that improves spectral precision and multiplexing in super-resolution microscopy using DNA Origami Nanorulers and spectroscopic SMLM.

The paper identifies emperipolesis as a process where megakaryocytes transfer proteins to neutrophils, enhancing their immune function and migration.

This study identifies distinct roles for CetZ1 and CetZ2 in shaping Haloferax volcanii cell morphology during different growth phases, showing CetZ2 maintains plate shape in stationary phase by counteracting CetZ1's rod-forming activity.

The paper introduces SAKe, a designer protein that forms stable, pH-dependent two-dimensional assemblies for functional protein-based materials.

This study shows that MICOS assembles on non-respiring mitochondrial precursor cristae in trypanosomes and identifies new associated proteins.

The paper visualizes nanopores in endothelial cells in situ using a super-resolution microscope after staining with a fluorescent dye.

This study reports cryo-EM ribosome structures and rRNA modifications in Babesia divergens, revealing novel modifications with potential therapeutic implications.

The paper introduces Mega-expansion microscopy, a fluorescence imaging technique that enables high-resolution structural visualization of whole cells using conventional microscopes.

This study shows that stress granule fusion is coordinated by mitochondria, enhancing cell viability and stress adaptation.

The paper identifies an essential Ndc10 trimerization interaction in yeast kinetochores that stabilizes centromeric DNA and facilitates kinetochore assembly.

This study investigates BCoV spike protein evolution, showing preserved architecture but functional region remodeling, with implications for vaccine development.

This study shows pathogenic bacteria use extracellular vesicles to alter host membrane mechanics, preventing phagosome maturation and aiding infection.

This study investigates how Myomerger clusters of different sizes influence membrane remodeling during myoblast fusion, showing size-dependent effects on membrane tension and lipid compaction.