flow cytometry

This study shows that gut microbiota influences liver pathology caused by Schistosoma mansoni infection in mice, but not ileum inflammation.

The paper profiles the immune system of elderly individuals and identifies systems-level signatures of aging, sex, smoking, and clinical traits using high-dimensional flow cytometry and multi-block analysis.

This study shows that timing anti-PD-L1 therapy after radiation enhances tumor response by preventing CTL depletion and improving PD-L1 blockade.

This paper introduces a semi-automated protocol for isolating multiple liver cell types from mouse and human livers with high yield, purity, and viability.

This study compares viral and non-viral methods for CAR T cell engineering, finding that non-viral SB transposition alters T cell activation and co-inhibition markers, with CAR design and donor type influencing outcomes.

This study shows that SLAMF6 is essential for CD8+ T cells to form synapses and kill HIV-1-infected CD4+ T cells, as blocking SLAMF6 reduces conjugate formation and cytotoxicity.

This study shows matrix rigidity enhances M2 macrophage activation by altering nuclear mechanics and chromatin accessibility through cooperation with biochemical signals like IL4/13.

The paper tests four neutrophil depletion protocols in neonatal mice, finding that the P3 Dual protocol achieves the best neutrophil clearance with minimal ethical concerns.

This study develops a 37-marker spectral flow cytometry assay for high-dimensional immunophenotyping of cryopreserved liquid biopsies and validates its robustness against cryopreservation effects.

This study shows that human T follicular helper cells differentiate in vitro through STAT3 and SMAD cytokine cooperation, but IL-2 inhibits this process.

This study develops an in vitro lymph node microphysiological system to model early T cell-B cell interactions and antibody responses using primary human lymphocytes.

This study shows that exofucosylation of T cells enhances their homing and therapeutic efficacy in treating solid tumors and metastases.

The paper develops a 30-color immunophenotyping panel to identify and sort immune cell subsets in autoimmune diseases using minimal samples and high resolution.

This study identifies CD20-expressing NK cells with enhanced functionality, memory-like features, and enrichment in inflammatory diseases.

This study compares the immunomodulatory effects of hypoxia-preconditioned bone marrow and Wharton's jelly mesenchymal stem cells in acute graft-versus-host disease, finding WJ-MSCsHYP more effective due to enhanced mitochondrial transfer and metabolic reprogramming.

This study shows maternal type 2 immunity, independent of antigen exposure, transfers protective immunity to offspring via microchimeric cells.

This study shows pristane-induced lupus mice have increased activated neutrophils, LDGs, and NETs compared to controls, suggesting early innate immune activation contributes to lupus development.

The paper examines circulating PD-1hi CXCR5- and CXCR5+ CD4 T cells in newly diagnosed GCA patients and finds they predict relapse.

The paper investigates immune correlates of early Mtb clearance in TB household contacts, finding that BCG vaccination is linked to enhanced innate immune responses rather than adaptive immunity.

This paper standardizes fluorescent analysis of extracellular vesicles using nanoparticle tracking analysis and nano-flow cytometry with reference materials, enabling accurate quantification of marker abundance and heterogeneity.

The paper describes methods for studying graft-versus-leukemia immunity in xenogeneic mouse models using human AML cells and PBMCs.

This study compares the immunological effects of photon and proton radiation therapy in cancer patients, finding that proton therapy preserves T cell diversity and may improve survival.

This paper introduces a method using ultrasound-driven microbubbles to manipulate and sort human cells in microfluidic channels while maintaining cell viability.

This study examines long-term immune changes post-COVID-19 and the impact of BCG vaccination and latent infections on disease severity.

This study develops an in vitro differentiation and cell sorting method to isolate highly pure mouse basophils (>98%) that maintain lineage markers and functional responses.

This paper optimizes flow cytometry procedures to accurately identify, quantify, and purify extracellular vesicles and virus-like particles by reducing artifacts and improving specificity.

This paper introduces a microfluidic platform that deterministically pairs and sorts droplets containing cells, achieving high recovery of target cell pairs for studying cell crosstalk.

The paper generates and characterizes primary human cytotoxic T cells with suppressed function in vitro, showing their resemblance to exhausted tumor-infiltrating CTL and identifying factors affecting their effector function.

This study shows that IL-1R2 on Tfr cells prevents excessive activation and allergy by limiting IgE and promoting IgG responses.