flow cytometry

The paper quantifies cell dynamics and interactions in neuroblastoma cell cultures under stress, finding that cell-to-cell interactions drive phenotypic adaptation, which accelerates with lower nutrient levels.

This study shows LSD1 inhibition enhances antitumor immunity in HNSCC by upregulating MHC-I and activating the IFNγ-CXCL9-CXCR3 axis through dendritic cells and CD8+ T cells.

The paper develops a 17-parameter spectral flow cytometry panel to profile Th1, Th2, and Th17 cytokine signatures in murine splenocytes under steady-state and stimulated conditions.

This paper provides a step-by-step framework to analyze immunometabolic states of primary murine ILC2s and link them to phenotypes and functionality.

This study uses machine learning to predict acute Graft-versus-Host-Disease (aGvHD) based on immune cell and cytokine profiles at engraftment, finding high accuracy with cytokine and T-cell data.

This study investigates the functional differences between thymic and peripheral Treg cells in visceral adipose tissue, showing that tTreg instability contributes to inflammation and obesity while pTreg cells protect against metabolic disorders.

This study shows that changing antibody administration route and fluorophore improves leukocyte labeling efficiency, sensitivity, and duration for tracking migration.

This study shows that hepatocyte-specific cytochrome c oxidase deficiency in mice causes lymphopenia due to bone marrow progenitor defects and early autoimmunity.

The paper quantifies plasma membrane and whole-cell RTK expression in cultured human endothelial cells from different organs, finding organ-specific distributions that may not fully reflect angiogenic capacities in vitro.

This study identifies immune profiles in pediatric cerebrospinal fluid to differentiate pediatric-onset multiple sclerosis from other inflammatory neurological disorders.

This study explores using cMPL as a target to selectively deplete human hematopoietic stem cells, showing potential for safer pre-transplant conditioning.

The paper uses mass cytometry to identify immune cell signatures predictive of acute graft-versus-host disease (aGvHD) and shows how these signatures evolve over time.

The paper introduces a 22-color spectral flow cytometry panel to profile human PMNs for activation, degranulation, phagocytosis, and other responses using phorbol ester and bacterial challenges.

This study presents lineage tracing results from 18 myeloid-specific fate-mapping models in mouse peripheral blood and synovial tissue, validating synovial macrophage identification through flow cytometry and CITE-seq.

This study shows that NK cell receptor signaling and activation vary with cell cycle stages, with some proteins showing non-monotonic abundance changes.

The paper introduces a method to separate signal and noise in flow cytometry data to improve genome copy number estimation in E. coli.

This study evaluates a microwell system for isolating individual NK cells, demonstrating its viability and potential for functional screening but noting its limited throughput.

This study characterizes peritoneal exudate cells in liver cirrhosis patients using single-cell and bulk RNA sequencing, revealing shifts in immune cell populations and gene expression with disease severity.

The paper examines metabolic profiles of circulating plasmacytoid dendritic cells (pDCs) in systemic sclerosis (SSc) patients, finding differences based on clinical phenotypes.

This study investigates how stem cell transplantation (SCT) restores immune function in refractory Crohn's disease by analyzing immune cell remodeling and HSC heterogeneity using single-cell RNA sequencing and functional assays.

This study identifies lung CD4+ resident memory T cells and their interaction with airway secretory cells as key regulators of neutrophilic allergic airways disease in mice and humans.

This study examines sex-specific differences in CD4 Th subsets and lifestyle factors in a RA cohort, finding sex-related variations in Th cell frequencies and dietary patterns.

The paper examines monocyte subsets in Crohn's disease patients, finding classical monocytes show an exhaustion profile with increased CD38 and reduced IL-1β production after LPS stimulation.

This paper introduces UNITO, a deep learning framework for automated cytometric gating that matches human performance and provides interpretable results without requiring manual input.

The paper shows MAGE-A4 promotes NSCLC by recruiting IgA+ plasma cells, reducing tumor burden and enhancing T cell activity.

This study shows splenic MARCO+ marginal zone macrophages (MZMs) enhance cytokine production but are not required for anti-RBC alloantibody generation after stored RBC transfusion in mice.

This study examines the effects of antidepressants on immune responses and depressive behaviors in male and female CUMS mice, finding sex differences in T-cell activation and immune regulation.

This study shows cancer cells evade NK cell cytotoxicity by polarizing inhibitory ligands at the synapse via actin remodeling.

This study investigates whether MSC-EVs elicit an immune response in tumor-bearing mice and finds no significant immune activation, supporting their immune privilege and therapeutic potential.

This study identifies unconventionally primed TFH1 cells as essential for generating IgA+ long-lived plasma cells during mucosal viral infections.