flow cytometry

This paper introduces a CRISPR-based platform to study GABARAP proteins in autophagy, revealing their distinct associations with autophagosomes.

The paper examines how HIV exposure in infants affects maternal microchimeric T cells, finding reduced levels in CD8+ T cells among HIV-exposed uninfected infants.

This study shows hemin activates platelets and promotes thrombosis, which can be inhibited by ACKR3 agonists, suggesting a potential therapeutic strategy for atherosclerotic plaque vulnerability.

The paper shows CD4 downregulation precedes Env expression in HIV-1-infected cells, making them resistant to non-neutralizing antibody-mediated ADCC.

This study identifies early and delayed STAT1-dependent responses in splenic macrophages that drive trained immunity, enhancing protection against Salmonella Typhimurium infection.

The paper examines immunosenescence trajectories in diverse populations, finding associations between immunological age and brain atrophy, while highlighting CMV status and genetic ancestry as key factors.

This study shows that IL-21 enhances aNK cell expansion and cytotoxicity against tumors, with tumor lysate boosting their efficacy, validated in a zebrafish model.

This study identifies dysregulated antibody-mediated cellular responses in MIS-C, linking NK cell dysfunction to immune pathology and suggesting potential therapeutic interventions.

The paper demonstrates the use of double-emulsion droplet technology to analyze single-cell effector function in adoptive cell therapy, enabling high-resolution study of cell-cell interactions and functional heterogeneity.

This study shows ICOS limits the function of exhausted PD-1+ CD8 T cells by promoting their differentiation into less functional effector-like cells, and inhibiting ICOS improves anti-tumor responses.

The paper develops a 42-parameter mass cytometry panel for immunophenotyping leukocytes in rhesus macaque bronchoalveolar lavage, identifying multiple cell types and functional markers.

This paper introduces label-free ghost cytometry (LF-GC) for cell therapy manufacturing, showing it can quantify cell count, viability, and identify cell subsets and impurities without staining.

The paper investigates bovine dendritic-cell subsets in various tissues using flow cytometry and bulk RNA-seq, identifying potential cDC1, cDC2, and pDC subsets based on CD4 and CD13 expression.

The paper examines the expression of XCR1, CX3CR1, CSF1R, and ADGRE1 on bovine blood dendritic cell and monocyte subsets using flow cytometry and validates findings with bulk RNA-seq data.

The paper identifies a subpopulation of bone marrow macrophages that support AML growth by inhibiting T cells and are enriched in patients with early relapse.

This paper introduces a spectral fingerprinting method using full-spectrum cytometry to analyze synthetic algal-microbial communities, showing improved accuracy in species identification and community tracking.

This study shows that the time of day a virus infects an algal host affects viral particle production and bloom dynamics.

This study establishes an in vitro model using Raw 264.7 macrophage cells to simulate resolving macrophage polarisation, showing sequential shifts from M1-like to M2-like to Mres-like phenotypes.

This study identifies pro-inflammatory macrophages as key regulators of the PE-specific immune microenvironment, linking them to Th17 and CD8+ T cell activation and gMDSC suppression in placental tissues.

The paper examines how engrafted monocytes adapt chromatin and transcriptionally in the injured retina, showing shared and distinct features with host microglia.

This study compares T-cell responses between healthy donors and aGVHD patients to optimize PHA and IL-2 concentrations for in vitro T-cell proliferation studies.

The paper develops a flow cytometry method to analyze T cell receptor Vβ and phenotype simultaneously, identifying long-lasting CMV-related T cell expansions with consistent TIGIT and PD-1 expression.

The paper introduces PIP-Seq to study lung ILC activation after exposure to environmental toxins, identifying novel ILC subsets and activation pathways.

This study shows that boosting mice with mRNA vaccines in the ipsilateral or contralateral arm leads to equivalent immune responses and protection against SARS-CoV-2 Omicron.

The paper develops a flow cytometry protocol to quantify pollen in Papaveraceae species, revealing lineage-specific patterns in pollen-to-ovule ratio reduction.

This study identifies that high B cell infiltrates in NSCLC tumors, associated with tertiary lymphoid structures, negatively correlate with the tumor-reactivity of expanded TIL products.

This study shows shear stress increases platelet glycosidase activity, leading to glycosylation remodeling, platelet count decline, and microvesiculation in MCS patients.

This study examines changes in co-stimulatory and inhibitory receptors on monocyte subsets in visceral leishmaniasis patients before and after treatment.

This study shows that perinatal liver inflammation leads to long-term changes in myeloid progenitors through the CXCL10-CXCR3 axis, which may contribute to immune dysfunction in biliary atresia.