Microtubule and mitosis dynamics

This study identifies N2G as an F-actin bundler and kinesin-1 activator that mediates active microtubule-actin crosstalk, crucial for nuclear positioning.

The paper demonstrates that CDK5RAP2 can form minimal centrosome-like structures in vitro, recruiting and activating gamma tubulin ring complexes to generate microtubule asters, and identifies key features of centrosome function.

This study determines the structure of the ch-TOG-TACC3 interaction and shows that inhibiting it causes pericentriolar material fragmentation and delayed mitosis.

The paper identifies cohesin protection errors in hybrid mouse oocytes as a cause of female sterility, linking it to speciation.

This study uses simulations to show how directional mechanical forces affect the unbinding of the NDC80 complex and other MAPs from microtubules, revealing design principles for load-dependent behavior.

The paper shows that ecDNA inheritance is ensured through mitotic transcription and chromosomal tethering via BRD4 and PVT1, preventing cytosolic mis-segregation and chromosomal integration.

This study shows that centromeric chromatin is mechanically resilient and distinct from pericentromeric chromatin, which deforms under force, with CCAN components not affecting pericentromere stretching.

This study shows that centrosome components like Sas6, Cenexin, and DZIP1 promote Xvelo assembly into a Bb-like structure in somatic cells.

This study investigates separase inhibition and cohesin protection in oocytes, finding that cohesin is not protected early in meiosis I and at metaphase II arrest, and that kinetochore individualization in meiosis I is required for cohesin cleavage in meiosis II.

This study identifies TinA as an activator of KlpA, enabling minus-end-directed motility through direct interaction and structural modulation.

This study shows NuMA acts as a dynein adaptor, stabilizes microtubule minus ends, and facilitates their transport to spindle poles, with γTuRC hindering NuMA binding.

The paper identifies adaptation strategies in aneuploid cancer cells through in vitro evolution and multi-omics analysis, revealing key transcription factors and genomic changes that improve proliferation and survival.

The paper shows that Spd-2 recruits Polo and Aurora A to form two distinct scaffolds, Cnn-scaffold and TACC-scaffold, which are essential for mitotic centrosome assembly in Drosophila.

This study shows that Mis4 is essential for proper nuclear separation in fission yeast after quiescence, with implications for human diseases involving NIPBL.

This study identifies multiple CCAN binding sites and shows that FACT localization to kinetochores depends on specific CCAN subcomplexes and is regulated by CK2 phosphorylation and DNA displacement.

This study shows that cells lacking Bub3, a spindle checkpoint protein, retain specific chromosomes through aneuploidy, which provides a survival advantage by increasing the expression of genes involved in chromosome segregation.

This study identifies three mechanisms that maintain mother-daughter centriole engagement and describe how structural changes enable their disengagement during mitosis.

This study identifies Kinesin12-II as a motor protein essential for transporting cell plate materials to the phragmoplast midzone in moss, revealing its role in cell plate formation and gametophore development.

This study compares two FtsZ isoforms in Odinarchaeota, revealing distinct filament structures and membrane tethering mechanisms, highlighting evolutionary diversity in the FtsZ-Tubulin superfamily.

The paper shows LIN-5/NuMA regulates spindle orientation and tissue organization in C. elegans germ cells through polarized cortical localization.

The paper shows that separated N- and C-terminal parts of Ana1 can rescue centriole duplication but not elongation, highlighting distinct functional requirements for Ana1 in these processes.

This study investigates the phase separation of Bik1, a +TIP protein, revealing its conformational changes and fractal structure in liquid condensates.

This study investigates how macromolecular crowding agents affect microtubule polymerization rates and filament dynamics through experiments and simulations.

This study investigates SAC function and biorientation pathways in Drosophila meiosis I, finding SAC is not required for chromosome segregation but some SAC proteins are involved in biorientation.

The paper uses deep learning to restore high-resolution images of actin microfilaments in plant cells, revealing distinct localization patterns during early cell plate formation.

This study investigates the differential GTP-dependent polymerization of Physcomitrella FtsZ isoforms in vitro and their synergistic interactions.

This study shows that the Eg5 inhibitor SB743921 induces p53-dependent cell cycle arrest, senescence, and death in tumor cells, particularly in TP53-mutated cells, with potential for combinational therapy with senolytic drugs.

The paper uses cryo-ET to reveal the architecture of centromeric chromatin clearings and their role in kinetochore formation and amplification.

This paper develops a photo-responsive protein system to dynamically control microtubule superstructures using light.

The paper shows Aurora B regulates abscission speed in stem cells by controlling microtubule stability through a feedback loop.