Fungal and yeast genetics research

The paper analyzes 3,034 Saccharomyces cerevisiae genomes to characterize population structure, revealing 93% species diversity and ecological clustering patterns.

Saccharomyces Genome Database released two S288C annotation updates (R64.4.1, R64.5.1) adding ncRNAs, ORFs and uORFs, overhauled YeastPathways, and expanded search, GFF and web features.

The paper introduces an AID library for on-demand protein depletion in yeast, enabling systematic study of protein function and uncovering new roles in cell cycle and mitochondrial processes.

The paper designs and validates six minimal integrating shuttle vectors for Saccharomyces cerevisiae with reduced size and restriction sites outside the MCS.

This study identifies mitochondrial efficiency as the key factor determining the Crabtree effect in yeasts by comparing Pichia kluyveri and Saccharomyces cerevisiae.

CAYSS automates the analysis of yeast spore segregation data to measure recombination rates and interference from flow cytometry data.

The paper challenges a previous finding by showing that rpl1b deletion mutants are competent for respiration, contradicting the earlier report of a petite phenotype.

This study identifies IRA2 as a key gene for ethanol tolerance in Saccharomyces cerevisiae through QTL mapping and molecular validation.

The paper investigates how priority effects influence gene expression in nectar yeast, finding differential expression in amino acid metabolism and eQTL enrichment for antifungal resistance.

The paper links genome to proteome in yeast progeny to identify variants controlling proteome diversity and predict their fitness effects under stress.

The paper identifies a yeast survival mechanism called VRQT, where aged stationary-phase cells divide synchronously to enhance population survivorship during starvation.

The paper defines domain boundaries of K1-like killer toxins across yeast species and identifies conserved alpha domains with cytotoxic activity.

The paper shows that deleting BAP2 is lethal in a leucine-requiring yeast strain with fermentable sugars but not other carbon sources, and this lethality is overcome by deleting BUL1.

The paper shows that formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Pichia pastoris, enabling self-induced protein expression without methanol.

This study uses solid-state NMR to analyze the molecular structure of cell walls in Mucorales fungi, revealing the composition and organization of chitin, chitosan, and associated polysaccharides.

This paper introduces an improved tetracycline-inducible expression system for fission yeast that enables control of gene expression in non-dividing cells and supports synchronous meiosis induction.

The paper shows that NSF in fission yeast interacts with Hmt2 and shifts gene expression from fermentation to respiration-like, independent of carbon source.

The paper introduces pSPObooster, a plasmid system that improves sporulation efficiency in S. cerevisiae lab strains by correcting meiotic defects in MKT1 and RME1 genes.

This study examines how stress conditions alter the structure and function of membrane-based organelles in budding yeast using fluorescent markers.

This study identifies three gene clusters in Candida intermedia responsible for lactose and galactose metabolism, revealing a unique regulatory network distinct from other yeasts.

This study identifies beta-1,6-glucan as essential for fungal cell wall structure, integrity, and host-pathogen interactions.

The paper develops improved AMA1-based plasmids for more consistent and stronger gene expression in filamentous fungi, showing increased reporter expression, CRISPRa efficiency, and metabolite production.

This paper develops an efficient gene targeting system in Trichophyton rubrum using a Δku80 strain and shows that Cyp51A contributes to azole drug tolerance by responding to efinaconazole.

This study examines how temperature affects meiotic success and recombination rates in two yeast species with different thermal tolerances, finding temperature-dependent differences in sporulation and recombination.

The paper biochemically characterizes KpAspE, a Group 5 septin from Knufia petricola, showing it recapitulates functions of canonical septin hetero-octamers in vitro.

This study identifies the Rim/Pal pathway in Malassezia sympodialis as essential for alkaline pH adaptation and demonstrates its role in atopic dermatitis.

The paper shows that Pho85, a non-canonical CDK, initiates a pathway to restart the cell cycle after stress by phosphorylating Sch9 and Whi5, enabling cell survival.

The paper identifies yeast strains with enhanced recombinant laccase production and characterizes genetic factors contributing to this trait.

This study compares cell wall structure in Aspergillus nidulans and Aspergillus fumigatus using solid-state NMR, revealing similarities and differences in polysaccharide composition and organization.

This study identifies phosphate transporters in Candida albicans and shows how mutations in these genes affect growth and stress resistance under phosphate scarcity.