Pluripotent Stem Cells Research

This paper develops a method to rapidly generate ventral A9-like dopaminergic neurons from iPSCs using small molecules and Ascl1 expression, creating functional 3D organoids for Parkinson's disease research.

The paper shows that modulating the JAK2-STAT3 pathway enhances the yield and maturation of human iPSC-derived myogenic progenitor cells for potential cell therapy.

This study identifies fibrocytes as a reprogrammable somatic cell source for bovine iPSCs, showing their molecular identity and successful reprogramming into pluripotent stem cells.

This study uses electrical stimulation to enhance the maturation of human neuromuscular organoids, improving their structural and functional properties.

This study enhances iPSC-derived cardiomyocyte maturation by combining 3D culture with a metabolic medium, resulting in improved structural, electrophysiological, and functional characteristics.

This study shows that TET protein deficiency in mouse ESCs leads to impaired somatic differentiation and accelerated germline entry.

This study shows that low-dose GSK3 inhibitors prevent aneuploidy in hPSCs by reducing mitotic DNA synthesis and ultra-fine chromosome bridges during long-term culture.

This paper describes a protocol to generate parasympathetic neurons from hiPSC that exhibit native electrophysiological properties and modulate hiPSC-atrial cardiomyocyte activity.

This paper generates bovine formative embryonic stem cells with dual developmental plasticity, capable of differentiating into various embryonic and extraembryonic cell types.

This study maps dynamic lipid changes during human cardiomyocyte differentiation using lipidomics and transcriptomics, identifying key lipid species and their roles in cardiac development.

This paper presents a method to produce high-purity endothelial cells from human pluripotent stem cells without cell sorting, resulting in cells with arterial-like characteristics comparable to HUVECs.

This study evaluates variability in kidney organoid structure development across different culture approaches and iPSC lines using high-throughput methods.

This study evaluates TLR expression patterns in hESC-derived cardiomyocytes to assess their functionality and maturation.

This study evaluates inducible transgenic systems in human pluripotent stem cells and differentiated cells, identifying a small molecule-mediated splicing switch that enables tunable transgene expression without protein tags.

This study shows mTORC2 is required for BMP4-induced WNT activation and mesoderm differentiation through cell-cell interactions in hESCs.

This study shows that calcium regulates pluripotency, cell cycle, differentiation, and post-translational/post-transcriptional processes in mESCs through pCaMKII-dependent mechanisms.

This study develops lentiviral constructs to induce specific epithelial cell types in human colonoids, revealing that KLF4 can generate goblet cells and colonocytes, while NEUROG3 and NKX6-3 induce duodenal enteroendocrine subtypes.

The paper develops a cardiac spheroid model with monocytes to better mimic heart failure responses, showing improved functional and transcriptional changes compared to models without immune cells.

This study identifies KLF7 as a key inducer of human pluripotency, showing its role in reprogramming somatic cells and enhancing chemical resetting to naive pluripotent stem cells.

The paper identifies NACC1 as a regulator of totipotent-like cells in mouse embryonic stem cells through a feed-forward mechanism involving gene regulation and retrotransposon activity.

This paper identifies miR-98-5p as a let-7 family microRNA that enhances naive human PSC induction by repressing NR6A1 and activating BMP2/4 signaling, improving resetting efficiency to ~80%.

The paper identifies transcription factors for reprogramming human astrocytes to neurons using CRISPRa screens and scRNA-seq, showing INSM1's broad neurogenic activity and cofactor interactions.

This study shows that multi-cellular human liver organoids can mature induced pluripotent hepatocyte-like cells through endogenous signals, achieving hepatic marker expression and function similar to primary human hepatocytes.

The paper develops patient-derived iPSCs to model ALS-PDC and study environmental and genetic factors in neurodegenerative diseases.

This paper describes a method to generate valvular interstitial cells from human pluripotent stem cells, showing high transcriptional similarity to primary fetal VICs and recapitulating disease features in an in vitro model.

The paper introduces scTF-seq, a method to study TF dose-dependent reprogramming in mouse MSCs, revealing heterogeneity in cell fate and TF sensitivity.

The paper investigates the role of HERVK LTR5Hs elements in human preimplantation development, showing their cis-regulatory impact on the epiblast transcriptome and their essential role in blastoid formation through ZNF729 activation.

This study generates region-specific airway basal stem cells from human pluripotent stem cells by modulating the NOGGIN-BMP axis, showing distinct differentiation potentials for proximal and distal airway cell types.

The paper shows that mechano-osmotic changes in pluripotent stem cells influence chromatin state and fate transitions by integrating mechanical and biochemical signals.