Pluripotent Stem Cells Research

The paper shows that fluctuations in OCT4 and SOX2 levels influence cell fate decisions by affecting chromatin accessibility at differentiation enhancers.

The paper uses MINUTE-ChIP to analyze H3K27me3 and H3K4me3 in mouse ESCs under 2i and serum conditions, finding hypermethylation of H3K27me3 and loss of H3K4me3 at bivalent promoters.

This study identifies distinct roles of Pum1 and Pum2 in ESC pluripotency and embryogenesis, revealing novel post-transcriptional regulatory mechanisms.

The paper compares the interactome networks of human embryonic stem cell lines with their tissue of origin, finding limited transcriptome overlap but enriched network properties related to transcriptional activity.

This paper develops a statistical model to evaluate differences in pluripotency ratings of stem cell colonies by two experts, identifying shared and distinct features used in their assessments.

This study shows that CHD8 deficiency in human iPSCs increases cell proliferation and delays neural differentiation, potentially contributing to ASD pathophysiology.

This study shows that differentiated zebrafish muscle cells can be reprogrammed into endoderm-like cells in vivo through Sox32 and Oct4 co-expression, challenging previous assumptions about lineage restriction.

This study generates implantation-competent blastocyst-like structures (iBCs) from mouse pluripotent stem cells, demonstrating their ability to implant and develop in vivo.

The paper uses scRNA-seq to analyze graft composition in a Parkinson’s disease cell therapy model, revealing differences between human stem cell-derived and fetal tissue grafts.

This paper develops a standardized workflow for high-content screening of 3D human stem cell cultures using serum-free embryoid bodies to improve phenotypic drug screening efficiency.

This study identifies CD49f as a marker to isolate functional human iPSC-derived astrocytes, which can be stimulated to exhibit a neurotoxic phenotype relevant to neurodegenerative diseases.

This study shows that reducing EHMT1/2 activity during reprogramming with ascorbic acid impairs iPSC formation and viability, highlighting the role of H3K9 methylation in reprogramming efficiency.

This paper demonstrates that chimpanzee iPSCs can form cross-species chimeras with rhesus macaque embryos, suggesting functional similarity between human and chimpanzee cells.

The paper develops Reprogram-Seq to screen TF cocktails for reprogramming cell states, identifying a 3-TF combination that reprograms MEFs to epicardial-like cells.

This study identifies ZIC3 as a key regulator of the transition from naïve to primed pluripotency in mouse ESCs by analyzing open chromatin changes and transcriptional networks.

The paper analyzes in vitro NMPs derived from ESCs and EpiSCs, finding that EpiSC-derived NMPs more closely resemble in vivo NMPs and contain a node-like population maintaining T expression.

The paper analyzes cell cycle dynamics in human pluripotent stem cells primed for differentiation using RNA-sequencing and identifies PI3K signaling's role in early differentiation states.

The paper identifies piRNA expression during cardiac differentiation of human pluripotent stem cells and suggests their potential role in gene regulation during cardiomyocyte development.

This study investigates how Gata4, Mef2c, and Tbx5 drive cardiac reprogramming by analyzing transcriptomic and epigenomic changes in fibroblasts.

The paper shows that KLF4 protein stability, regulated by interactions with pluripotency factors, overrides transcriptional control in ES cells, and mutations affecting stability prevent differentiation.

The paper identifies RNA binding proteins and RNAs interacting with Sox2 in mouse embryonic stem cells, showing coexistence in a ribonucleoprotein complex without evidence of chromatin recruitment regulation.

The paper maps multi-omic changes during mouse pluripotency transition, showing phosphoproteome dynamics precede epigenome and transcriptome changes.

This study identifies reprogramming checkpoints in iPSC generation by analyzing single-cell transcriptomes and reveals key gene interactions and regulatory mechanisms.

The paper uses RNAi screens in isogenic human stem cells to identify SMARCA4 as a differential regulator of stemness and differentiation in hematopoietic and neural stem cells.

The paper identifies two distinct pluripotency states in mouse embryonic stem cells (mESCs) based on G2M phase enrichment, showing naive and formative states marked by Esrrb expression levels.

The paper shows that chemically-defined conditions and signaling molecules can generate primitive endoderm-like cells and support post-implantation-like epiblast development in vitro.

The paper identifies markers of naive and lineage-primed hematopoietic progenitors from hPSCs using single-cell RNA sequencing and functional assays.

The paper compares hypoxia responses in human and chimpanzee iPSC-derived cardiomyocytes, finding conserved transcriptional responses and species-specific regulatory differences.

This study identifies DPPA2 and DPPA4 as essential for activating Dux and LINE-1 transcription, which is critical for zygotic genome activation in mouse embryos.

The paper identifies Brca1, Bard1, and Wdr5 as factors linking DNA damage response to MET during reprogramming, using siRNA screening and colony morphology analysis.