Advanced Fluorescence Microscopy Techniques

The paper introduces a live-cell imaging method combining spectral linear unmixing and ratiometric FRET to quantify protein interactions and signaling dynamics with high spatial and temporal resolution.

FLEXTAG improves super-resolution imaging by enabling long-term, multi-color, low-bleaching labeling of subcellular structures in live and fixed cells.

The paper compares three CUBIC-based tissue clearing protocols in murine organs using volumetric fluorescence imaging to assess transparency and staining quality.

The paper develops a thermostable green fluorescent protein, Matcha, for live imaging in hyperthermophiles and uses it to study division ring dynamics in Sulfolobus.

This paper introduces FA-SIM, a super-resolution microscopy technique that quantitatively maps intracellular microenvironment and cytoskeletal dynamics with high accuracy and low phototoxicity.

PAVR enables high-resolution volumetric imaging of cells using a physics-aware framework trained on simulated data, demonstrating applications in tracking subcellular structures and analyzing cardiac cell dynamics.

This paper provides a protocol for building and characterizing an oblique plane microscope (OPM) to enable fast, multi-color, high-resolution volumetric fluorescence imaging with reduced phototoxicity.

This study shows fluorescence intensity decreases over six weeks in IHC, influenced by antibody choices and staining methods, highlighting the need for imaging best practices.

This paper investigates how absorption dipole orientation affects MINFLUX localization accuracy, finding that tilted dipoles introduce biases that depend on their orientation and position within the imaging area.

The paper develops a red fluorescent calcium indicator, ScaRCaMP, that is compatible with optogenetics due to its high blue-light photostability.

The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.

The paper introduces GCaMP8 variants targeted to synaptic compartments and develops CaFire software to quantify Ca2+ signals, showing improved sensitivity and resolution compared to existing methods.

DeepSRFusion improves super-resolution particle fusion for structural biology by addressing rotational perturbations and sparse labeling with a self-supervised framework, achieving high resolution and speed.

The paper develops an ultrasensitive ratiometric glucose sensor to monitor glucose transport and metabolism in plants, finding rapid hydrolysis of shoot-derived sucrose in root tips.

This study investigates residual polarization ellipticity in waveplate-compensated polarization-resolved SHG microscopy and identifies femtosecond laser spectral bandwidth as a potential cause.

This paper introduces Airy beam light sheet microscopy to image subcellular dynamics in large tissues with high spatial and temporal resolution across multiple organisms and biological processes.

The paper characterizes MINFLUX imaging performance over extended durations using DNA origami and corrects residual drift, achieving sub-2 nm precision without loss of site precision.

This paper introduces a single-step expansion microscopy method enabling SMLM with spontaneously blinking dyes in expanded samples, achieving nanometer-resolution imaging of cellular structures.

This paper develops a framework to quantify protein oligomerization in living cells using fluorescence lifetime and anisotropy imaging, validated with GPCRs and applicable to drug discovery.

This paper introduces TExM, a method to super-resolve fixed and live cellular samples by mechanically expanding hydrogels during continuous imaging.

This paper introduces BEEP learning, a machine learning framework for multi-view fluorescence unmixing that improves discrimination of fluorophores in multiplexed imaging.

The paper quantitatively evaluates nine green and eight red fluorophores in Drosophila embryos to identify optimal choices based on bleaching, folding, and imaging conditions.

This paper introduces a label-free, real-time single-molecule immunoassay using iSCAT to directly observe protein-protein interactions with high sensitivity and molecular-weight resolution.

This study investigates how PEG-induced phase separation affects FRET-based crowding sensors, showing that CrH2 forms puncta while CrD does not, and identifies biophysical mechanisms behind the sensor response.

BLIMPS enables high-speed, high-resolution imaging of presynaptic Ca2+ dynamics in both excitatory and inhibitory neurons using lattice light sheet microscopy and genetically encoded calcium indicators.

This paper shows how modifying the electrostatic environment of a fluorescent protein's beta-barrel can control chromophore fluorescence properties and response kinetics.

The paper introduces a mobile microscope system, CortexCAM, for imaging large areas of the mouse cortex during natural behaviors.

This paper develops a method to estimate the separation of two freely rotating dipole emitters near the quantum limit using vectorial theory and a modified SLIVER technique.

This study demonstrates that rhodopsin-based voltage indicators can be used for two-photon voltage imaging, achieving high-fidelity action potential detection in vitro and in vivo.

This paper introduces a method using mass photometry with supported lipid bilayers to detect and measure proteins in complex mixtures without prior purification.