Advanced Fluorescence Microscopy Techniques

This study demonstrates electrokinetic detection of single-molecule phosphorylation, resolving phosphorylated sites and monitoring kinase cycles with single turnover resolution.

This paper introduces a microscopy technique that enables 3D RNA imaging in thick brain sections by combining spatiotemporal localization with volumetric excitation, revealing detailed neuronal distributions and expression gradients.

This paper introduces a method to parallelize single-molecule binding kinetic measurements using protein barcode sequencing, enabling rapid and multiplexed screening of protein libraries.

The paper introduces a far-red dopamine sensor, HaloDA1.0, enabling multiplex imaging of neurochemical networks in vivo with high sensitivity and versatility.

This paper introduces a model-based method to estimate field-dependent aberrations in single molecule localization microscopy without calibration, improving localization accuracy across large fields of view.

The paper introduces novel far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging, enabling subcellular structure distinction without hardware modifications.

The paper introduces a parallelly distributed microscope and software system for high-throughput multispectral 3D imaging with high resolution and data throughput.

This paper introduces two photostable voltage sensors, ElectraON and ElectraOFF, which enable longer in vivo recordings by improving photostability compared to existing GEVIs.

This paper introduces a two-photon fiberscope with tunable focus for in vivo imaging at selected depths, demonstrating its performance using phantoms and biological samples.

This paper develops an implantable microprism interface for deep-brain imaging with submicrometer resolution and wide-field two-photon microscopy.

The paper demonstrates prolonged single-neuron voltage imaging in behaving mice using ElectraOFF, revealing plasticity changes during neuromodulation.

This paper optimizes genetically encoded nanoparticle expression to improve single-particle tracking of cytoplasmic diffusion in mammalian cells, showing increased diffusivity and better tracking quality.

This paper introduces a DNA-PAINT system with optical photon reassignment on a spinning disk confocal to achieve high-resolution imaging in thick samples and large fields of view.

The paper introduces Vega, a photostable GEVI with high sensitivity and fast response, enabling high-fidelity voltage imaging in tissue and live animals.

FLIMPA is an open-source software for FLIM phasor analysis that integrates data from multiple trials and provides tools for quantifying microtubule depolymerisation.

This paper demonstrates three-photon in vivo imaging in the mouse medial prefrontal cortex to achieve sub-cellular resolution of neuronal and glial activity at unprecedented depths.

This paper introduces GLF-MINFLUX, a method for nanoscale imaging of densely packed structures, achieving improved localization precision and faster acquisition.

This paper introduces a method to image neuronal voltage with super-resolution beyond tissue scattering limits, achieving high precision in dense neuronal populations.

This paper introduces near-zero photon imaging, a method that captures high-fidelity biological images at ultralow irradiance and high speed using AI.

This paper introduces a triple-labeling strategy for single molecule FRET to study conformational changes in GPCRs, revealing a previously unknown intermediate state.

DeepTRACE is a machine learning tool that analyzes single-molecule tracks in living cells, uncovering molecular processes and relationships not present in training data.

The paper uses a multimodal imaging approach to study breast cancer cell invasion in 3D spheroids, quantifying changes in metabolism, fatty acid storage, and collagen organization over time.

The paper develops IntAct-U-ExM to visualize actin networks with super-resolution, showing improved resolution of actin structures in yeast and mammalian cells.

This paper introduces a spectral CSD-ISM system for high-resolution, multi-color imaging with improved speed and resolution.

PerfectlyAverage determines optimal averaging parameters in laser scanning fluorescence microscopy by analyzing SNR, photobleaching, and PSD to reduce acquisition time.

This paper introduces SiLM, a chip-based 3D single-molecule localization microscopy method that achieves high axial precision with a single-objective lens, comparable to 4Pi methods.

This paper introduces a method to remove defocus backgrounds in fluorescence microscopy using dark channels and dual frequency separation, significantly improving image quality.

The paper introduces four new fluorescent protein fusions for Dorsal to study its dynamics in fruit fly embryos.

This paper develops a probabilistic algorithm to accurately identify and quantify intermolecular interactions using single-molecule localization microscopy.