Advanced Fluorescence Microscopy Techniques

This study shows that polyacrylamide hydrogels support DNA-PAINT imaging by preserving nanoscale docking strand positioning and enhancing diffusion and accessibility.

The paper introduces CoSiDeX, a tool for selecting and decoding fluorescent protein palettes in multiplexed imaging experiments using hyperspectral data.

This paper introduces a machine learning-based 3D SMLM method called easyZloc that works with standard microscopes and reduces computational demands.

This paper introduces VLM, a super-resolution microscopy method that combines hardware and algorithmic improvements to achieve high-fidelity 3D single-molecule imaging with 10 nm and 25 nm localization precision.

This paper infers binding and unbinding rates from fluorescence time series while accounting for photobleaching using a two-stage Bayesian approach.

This study uses FRAP-SR to reveal subcompartments within 53BP1 foci, showing differential protein mobility and function in different foci types.

The paper compares monomeric StayGold variants using protein nanocages in living cells, finding mStayGold is 3-fold brighter and more photostable than EGFP and other variants.

This paper introduces a low-cost FPGA-based method to correct pile-up distortions in TCSPC-FLIM, enabling high-speed, accurate in vivo imaging with subcellular resolution.

This paper introduces an image-based 3D active stabilization method for super-resolution microscopy, achieving ~1 nm stability without requiring fiducial markers or complex hardware.

The paper introduces GEQO biosensors for absolute analyte quantification in single cells without requiring specialized equipment, showing they account for buffering and retain time trace information.

This paper introduces a hierarchical adaptive optics strategy to improve three-photon imaging in the mouse brain during behavior by correcting optical aberrations at multiple levels.

This paper corrects optical aberrations in GRIN lens-based microendoscopes to improve imaging performance in deep brain regions.

This paper develops a framework for simultaneous particle tracking, phase retrieval, and PSF reconstruction to improve 3D tracking of lytic granules in T cells.

The paper introduces a preprocessing pipeline for 2-photon calcium imaging to improve subcellular signal detection and ROI grouping.

This paper develops a method to measure protein-protein interactions in real-time using cell-free synthesis and fluorescence correlation spectroscopy, achieving high sensitivity and throughput.

I2SIM improves axial resolution in 3D-SIM by using interference and spectral optimization, achieving 130 nm resolution for subcellular structures.

This paper introduces Meta-rLLS-VSIM, a super-resolution microscopy technique that achieves near-isotropic resolution with reduced training time and data requirements.

The paper develops a method to use mEOS-based reporters for membrane-associated AAA+ unfoldases by covalently linking mEOS to a degron via Sortase, preserving fluorescence and enabling functional analysis.

This paper introduces a mesoscopic SCAPE microscope with a super-oblique light sheet and rescanning strategy, achieving high-resolution volumetric imaging over large fields of view.

The paper introduces a method to map molecular complexes using IRIS super-resolution imaging and PC-coloring, revealing laminar complex formation in clathrin-coated structures upon EGF stimulation.

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

This paper introduces an open-source 3D active stabilization system for fluorescence microscopy to maintain sample stability during long imaging sessions.

The paper develops high-performance green and red fluorescent biosensors for pyruvate with large fluorescence intensity changes and varying affinities, enabling multicolor imaging in mammalian cells.

The paper introduces ABDS, an inexpensive open-source dye for eukaryotic cell visualization, and provides its preparation method and usage examples.

This paper introduces Hi-SMdM, a high-throughput single-molecule diffusivity microscopy approach that enables unbiased mapping of spatiotemporal heterogeneities in intracellular diffusivity with improved resolution and throughput.

The paper introduces four new monomeric fluorescent proteins for bacterial time-lapse imaging, showing improved photostability, brightness, and monomericity compared to existing standards.

The paper introduces AutoDS and AutoDS3D, deep learning tools for single-molecule super-resolution microscopy that reduce manual intervention and computation time.

The paper introduces a curved light sheet microscope for high-throughput imaging of cleared tissues, achieving diffraction-limited resolution over a large field of view.

Alpha-BET uses AlphaFold to identify optimal sites for labeling viral glycoproteins with nanobodies, enabling high-resolution imaging of viral structures without disrupting their function.

The paper introduces NEMOer, a set of genetically encoded calcium indicators that enhance the detection of ER/SR calcium dynamics, enabling sensitive and dynamic imaging in both excitable and non-excitable cells.