Advanced Fluorescence Microscopy Techniques

This paper introduces a single-objective lattice light sheet microscopy platform for improved 3D single-molecule super-resolution imaging in scattering samples.

This paper develops low-cost 3D printed lenslet arrays for super-resolution microscopy, achieving resolution comparable to commercial optics.

This paper demonstrates reduced signal contamination from out-of-focus cells in vivo voltage imaging using pHiLo compared to traditional widefield methods in awake mice.

The paper introduces FACED 2.0, a high-speed imaging technique that enables simultaneous voltage and calcium activity recording in vivo across large neuronal populations.

The paper evaluates MINFLUX performance using a simulator to identify limitations from fluorophore dynamics, background, estimators, and misalignment.

This paper identifies fluorophore blinking as a source of errors in MINFLUX imaging and demonstrates strategies to suppress these errors, enabling near-infrared MINFLUX with nanometer precision.

The paper introduces a method to improve deep imaging of expanded tissues using inverted microscopes by using FEP film and water immersion objectives, achieving sub-micron resolution and 800 µm depth.

This paper introduces a method for cryogenic light microscopy with Angstrom precision to study membrane proteins in near-native conditions.

This study develops a label-free optical method to assess metabolic function and oxidative stress in 3D brain tissue models using spectral imaging and fluorescence lifetime analysis.

This paper optimizes deconvolution methods for STED and CSLM microscopy to improve colocalization analysis of A- and B-type nuclear lamins in fibroblasts.

The paper develops all-optical strategies to reduce photo-bleaching in reversibly switchable fluorescent proteins, enhancing imaging resolution and duration.

This paper introduces a high-throughput miniature two-photon microscope for imaging calcium dynamics in freely moving mice, enabling large-scale recordings of over 1000 neurons in specific brain regions.

This paper accelerates fluorescence lifetime imaging (FLIM) using single-objective light-sheet microscopy, achieving faster and less phototoxic imaging of live multicellular systems.

This paper introduces a compact two-photon imaging module that enables simultaneous dual-depth, multi-region in vivo brain imaging, revealing context-dependent and depth-divergent neural activity patterns.

The paper uses smart 3D super-resolution microscopy to study the RNA scaffold structure in paraspeckles, revealing new insights into their 3D organization and growth.

This paper introduces pan-ExM-t, an all-optical method for visualizing specific proteins in the ultrastructural context of brain tissue, demonstrating its application on various markers in mouse brain sections and neuron cultures.

This paper introduces a deep learning method to improve calcium imaging by reducing scattering and increasing throughput in light-field microscopy.

SpeedyTrack enables microsecond wide-field single-molecule tracking and super-resolution mapping using EM-CCD vertical shift without modifying existing microscopy setups.

SurFlex microscopy quantifies flexibility of surface-tethered biomolecules using fluorescence anisotropy, revealing sequence-dependent DNA flexibility and changes in glycan flexibility on cell surfaces.

The paper describes a new calcium biosensor, G-Ca-FLITS, that maintains high brightness in both calcium-bound and -unbound states, enabling more accurate and consistent measurements.

This paper evaluates genetically encoded voltage indicators using FLIM to measure absolute membrane potential, finding that rEstus-NI provides the highest sensitivity and accuracy.

This paper introduces a method to monitor multiple bacterial metabolites in real-time using engineered periplasmic binding proteins in E. coli cultures.

The paper introduces a parameter optimization strategy for iterative MINFLUX microscopy to improve single particle tracking of fast lipid diffusion in membranes.

The paper introduces qMaLioffG, a green fluorescent protein FLIM indicator that quantitatively measures endogenous ATP levels by detecting fluorescence lifetime changes in physiological ATP concentrations.

This paper introduces a high-speed light sheet microscope with isotropic sub-micron resolution for cleared tissue imaging, achieving 850 nm resolution across 1 cm3 samples.

This paper develops two red-FRET ERK biosensors, REKAR67 and REKAR76, which operate in the 670-720 nm range and are spectrally compatible with existing biosensors for single-cell ERK activity measurement.

This paper describes a novel titanium imaging chamber for chronic optical recordings in marmosets, demonstrating long-term two-photon imaging quality and feasibility of voltage sensor imaging.

The paper presents a miniaturized widefield microscope for high-speed voltage imaging in freely moving animals, achieving a peak-to-noise ratio >3 at 530 Hz.

This paper introduces SP-SMLM, a hyperspectral and super-resolution imaging method that maps lipid membrane polarity with high spatial and temporal resolution in live cells.

The paper introduces a miniaturized micro-camera array microscope (mini-MCAM) for wide-field calcium imaging in freely behaving mice, enabling cortex-wide neural activity monitoring.