Bacterial biofilms and quorum sensing

This study tracks Pseudomonas aeruginosa evolution in 9 cystic fibrosis patients over decades, finding phenotypic convergence despite genomic divergence and reduced virulence features.

The paper uses time-lapse mesoscopy to study dual-species biofilms of Candida albicans and Staphylococcus aureus, showing that C. albicans morphology affects biofilm structure and infection synergy.

The paper identifies highly specific LasR variants for HSL signals through mutagenesis and screening, improving signal discrimination in microbial consortia.

This study examines how mutations in polyphosphate metabolism affect biofilm, capsule formation, and virulence in hypervirulent Klebsiella pneumoniae using a model host.

This study identifies non-canonical DNA structures like G-quadruplex and Z-DNA in staphylococcal biofilms that protect against DNase I and confer peroxidase activity to eDNA.

This study shows bacteria colonies adjust their mechanical properties based on agar substrate concentration through shear and compressive rheology measurements.

The paper identifies two compounds that inhibit quorum sensing in Pseudomonas aeruginosa by inducing the MexEF-oprN efflux pump, reducing QS-regulated virulence factors.

This study shows that elevated polymyxin B concentrations induce biofilm-specific resistance in V. cholerae through DNA release from lysed cells, forming a protective barrier.

This study identifies NahK as a regulator of pyocyanin production in Pseudomonas aeruginosa through the PQS system, showing its role in quorum sensing and virulence.

This study identifies shared inner membrane transporters for PQS-Fe3+ and PCH-Fe3+ in P. aeruginosa and shows their role in iron uptake and virulence.

This study shows that Candida albicans enhances Staphylococcus aureus virulence by inducing new, more aggressive colony variants through pH neutralization.

This study shows arginine reduces E. faecalis biofilm formation, alters gene expression, increases aggregation, and decreases susceptibility to antibiotics.

This study develops and benchmarks coculture microcolony experiments to quantify interspecific interactions and growth kinetics in bacterial communities.

The paper shows that bacterial motility enhances contact-based toxin delivery by increasing inter-strain contact and target switching, improving CDI effectiveness up to 10,000-fold.

This study identifies shared transcriptional pathways in two biofilm models of Vibrio fischeri, revealing genes involved in exopolysaccharide production and flagellar motility regulation.

This paper identifies MpaR as a cyanide-responsive regulator controlling ccoN4 expression in Pseudomonas aeruginosa, revealing a novel mechanism where cyanide acts as a signaling molecule to regulate gene expression.

This study introduces a new fixation method using alcian blue to preserve biofilm structure and immune cell interactions for SEM imaging.

The paper develops synthetic HSL sensors in Bacillus subtilis using LuxR-type regulators and combinatorial promoters, achieving up to 293-fold activation and identifying sequence-function relationships.

This study identifies three distinct modes of RhlR activation in Pseudomonas aeruginosa by analyzing how C4-homoserine lactone and PqsE influence RhlR binding and transcription.

This study investigates the role of the Entner-Douderoff pathway in Pseudomonas aeruginosa during catheter-associated urinary tract infections, finding it dispensable in this model.

The paper identifies PfsE as a Pf phage protein that inhibits PQS quorum sensing and type IV pili extension, enhancing viral replication and protecting the host from competing phages.

This study shows that calcium ions interact with alginate and extracellular DNA in Pseudomonas aeruginosa biofilms to alter their structure and protect against neutrophil phagocytosis.

The paper develops a spatiotemporal model of antibiotic accumulation in P. aeruginosa biofilms that replicates periphery sequestration observed in experimental studies.

This study shows that varying RecA levels in Acinetobacter baumannii affect biofilm formation and dispersal through the regulator bfmR, highlighting the role of DDR in infection persistence.

The paper tracks the rise in lasR mutations in Pseudomonas aeruginosa keratitis isolates from 1993 to 2021, showing increased frequency and association with worse clinical outcomes.

This study shows that the Rgg2/Rgg3 quorum sensing system is essential for Streptococcus pyogenes to establish and maintain skin infections in a mouse model.

The paper investigates the molecular targets of Silversol, a colloidal nanosilver formulation, in multidrug-resistant P. aeruginosa, identifying key genes involved in nitrogen metabolism and denitrification as potential targets.

This paper identifies LAI-1 as a signaling molecule secreted by Legionella pneumophila via outer membrane vesicles, promoting inter-bacterial and inter-kingdom communication.

This study identifies bacillaene, a metabolite from Bacillus subtilis, as an anti-biofilm compound that inhibits E. coli curli amyloid fiber assembly, revealing its dual antibiotic and anti-curli functions.

This study identifies 273 genes associated with biofilm formation in Listeria monocytogenes through GWAS and 220 genes through RNAseq, finding fewer overlaps than expected, suggesting distinct evolutionary adaptation mechanisms.