Advanced Electron Microscopy Techniques and Applications

DeepTracer predicts protein backbone atoms, secondary structures, and amino acid types from cryo-EM density maps with high accuracy and speed.

The paper introduces a graph-based method to build atomic protein models from cryo-EM density maps using residue co-evolution data to improve segmentation and sequence assignment.

The paper improves a marker-free image alignment method for electron tomography to enhance 3D reconstruction quality by addressing deformations during acquisition.

The paper introduces a user-guided refinement approach for subtomogram averaging to improve alignment of structurally complex macromolecular assemblies.

This paper introduces enhanced FIB-SEM technology that significantly increases imaging volume and resolution, enabling large-scale connectomics and cell biology studies.

The paper uses cryo-tomography to model the actin core structure of mouse vestibular hair cell stereocilia lacking plastin 1, revealing structural features and gaps in actin filament organization.

The paper presents a pipeline for cryo-EM image processing of microtubules using RELION, enabling accurate determination of microtubule architecture and seam location with near-atomic resolution.

This paper introduces an automated method for selecting density thresholds in cryo-EM maps to improve backbone structure prediction accuracy.

This paper solves the first unknown protein structure using MicroED, demonstrating its potential for discovering novel protein structures.

This paper describes a cost-effective photoconversion setup for converting fluorescent signals into electron dense ones, improving workflow reliability and throughput for neuronal tissue analysis.

MagC enables the remote collection of ultrathin sections for volumetric correlative light and electron microscopy by using magnetic resin, improving reliability and reducing manual effort.

The paper determines the 4.0 Å resolution structure of CFA/I pili and confirms the role of proline 13 in structural stability using optical tweezers.

AutoCryoPicker is an unsupervised method for automated single particle picking in cryo-EM images, improving accuracy and reducing bias compared to manual or supervised approaches.

The paper solves a high-resolution structure of a stalled RNA polymerase II elongation complex using streptavidin affinity grids to overcome air-water interface interactions.

The paper introduces a genetic iron-biomineralizing nanocompartment for multiplexed EM gene reporter imaging in mammalian cells.

This paper introduces a human-in-the-loop framework for improving EM image quality through interactive image restoration and analysis.

This paper introduces a high-throughput TEM imaging platform with automated serial sectioning for generating detailed biological tissue datasets, particularly for brain circuit analysis.

This paper introduces a new cryo-EM system for electron 3D crystallography called eEFD, showing improved data quality compared to 200 kV systems without energy filtration.

This paper develops a method to rapidly freeze unfixed stereocilia for 3D imaging, revealing structural features of stereocilia in a near-native state.

The paper discusses the removal of a manuscript due to a ruling by ETH Zurich.

The paper provides a protocol for preparing human tissue samples for multibeam scanning electron microscopy to enable nano-to-mesoscopic mapping of cellular connectomes and their habitats.

The paper introduces a Bayesian scoring function to rank structural models using cryo-EM data and other information, validated on known complexes and implemented in an open-source platform.

The paper introduces CoLSSEM, a method combining fluorescent labeling and electron microscopy to visualize synaptic connections in single neurons in vivo.

The paper introduces new computational tools for validating cryo-EM maps and atomic models, including methods for resolution estimation, map sharpening, and map analysis.

FluoEM identifies multi-color fluorescent axons in 3D EM data without fiducial marks by matching neurite reconstructions from light microscopy.

The paper benchmarks cryo-EM single particle analysis workflows using a standard sample and procedures to ensure high-resolution map production.

This paper introduces a method to simultaneously determine protein structure and dynamics from cryo-electron microscopy data, applied to the STRA6 receptor.

The paper describes new features and applications of Spotiton, a cryoEM sample vitrification instrument, showing it produces high-quality specimens with no apparent sample damage.

The paper demonstrates that small proteins can be visualized at near-atomic resolution using cryo-EM when attached to a symmetric protein scaffold.

The paper explores using crowdsourcing for cryo-EM particle picking, showing its potential and limitations through experiments with untrained citizen scientists.