CRISPR and Genetic Engineering

This study evaluates C. elegans U6 promoters' impact on CRISPR/Cas9 gene editing efficiency, identifying four superior promoters that enhance editing outcomes.

The paper identifies cognate recombination directionality factors (RDFs) for large serine recombinases using AlphaFold2-multimer and experimental validation.

This study compares deep mutational scanning and CRISPR base editing for variant function annotation, finding strong correlation between base editor data and gold standard DMS results.

This paper introduces snuABE, a single-nucleotide resolution ABE that reduces bystander effects through engineered ADAR and tagRNA design, achieving precise A-to-G editing in human cells.

This study characterizes EsAgo, a prokaryotic Argonaute from Exiguobacterium sp. AB2, showing it can cleave DNA with or without guides and has potential biotechnological applications.

This study uses SYNCAS to achieve efficient CRISPR-Cas9 gene editing in Nasonia vitripennis, with KO efficiencies up to ten times higher than other maternal injection methods.

This paper describes a CRISPR-based gene drive targeting the doublesex gene in Culex mosquitoes to suppress populations through male-biased propagation and sterile intersexes.

The paper introduces IntAC, a CRISPR screening method for Drosophila cells that improves accuracy by suppressing early Cas9 activity, leading to a more comprehensive list of fitness genes.

This paper enhances the Eco1 retron editor for precise genome editing in human cells by improving ncRNA stability and reducing sgRNA extension, achieving editing without double-strand breaks.

This paper introduces a synthetic DNA transduction platform that links DNA repair activity to CRISPR-Cas12a activation, enabling rapid and sensitive detection of DNA glycosylase activity and inhibitor screening.

Populus VariantDB v3.2 improves CRISPR and functional genomics research by enabling precise gRNA and primer design through querying sequence polymorphisms in poplar genomes.

This paper develops a CRISPR/Cas9 system with dual-sgRNAs for efficient large-fragment gene deletion in Mycobacterium abscessus, achieving over 90% deletion efficiency at certain loci.

The paper evaluates CasX2Max, a modified CRISPR system, showing it enhances cleavage efficiency for the CCR5 gene compared to native CasX2.

This paper introduces a method to modulate CRISPR activity in response to RNA detection using engineered guide RNAs, demonstrating functionality in mammalian cells and zebrafish embryos.

The paper identifies CRISPR-Cas12a variants with expanded PAM recognition, enabling targeting of ~25% of the human genome.

The paper develops a method for marker-free gene deletion in Trichoderma atroviride using CRISPR/Cas9 RNPs and transient telomere vectors, achieving efficient gene deletions via NHEJ.

This study develops efficient genome editing tools in Bacillus subtilis using IscB and enIscB, achieving high deletion and integration efficiencies.

The paper uses deep mutational scanning to identify Cas1 and Cas2 variants that enhance spacer acquisition in CRISPR-Cas systems, improving immunity against phage infection.

This study shows that CovR, a virulence regulator, enhances CRISPR-Cas9 immunity in Group B Streptococcus by modulating Cas9 activity and improving immune memory.

This paper simplifies the LACE optogenetic system from four to two plasmids, showing similar performance with reduced variability.

This paper demonstrates the use of Mpa Ago for in vitro mRNA processing and analysis, including 3' end polishing and comprehensive assessment of mRNA features using LC-MS/MS.

This paper introduces a method to temporally control CRISPR-Cas9 activity using bio-orthogonal chemistry, demonstrating its effectiveness in live cells and therapeutic applications.

CRISPRware designs context-specific gRNAs using sequencing data, enabling allele-specific targeting across the genome for gene therapy applications.

The paper presents a collection of minimal Pol III promoters for CRISPR applications, showing they can match longer promoters' performance in dicots.

This study uses guide RNA truncation and cryo-EM to visualize multi-turnover Cas9 states, revealing how PAM-proximal product binding inhibits re-binding of new targets.

The paper develops a method to quantify genetic interactions from CRISPR-Cas9 screens and identifies systematic variations in screen data.

This study uses CRISPR/Cas9 to achieve germline gene knockout in the subsocial wasp Sclerodermus guani, revealing a white-eye phenotype in homozygous mutants.

This study shows that crispant founder mice can be used for phenotypic assessment despite genetic mosaicism, as observed null phenotypes were present in up to 100% of founders.