Bacteriophages and microbial interactions

The paper characterizes the maturation of temperate phages in the infant gut microbiome and finds that viral microdiversity, not abundance, better predicts age, with stunting not delaying maturation.

The paper identifies cosmopolitan gut bacteriophages that expand the functional repertoire of health-related bacteria across diverse populations.

This paper develops an Fc-fused lysin, Fc-LysM-CHAP, optimized for improved pharmacokinetics and synergy with antibiotics against S. aureus infections.

This study isolates and characterizes four broad-range Staphylococcus epidermidis phages, identifies host resistance mechanisms, and highlights their potential for phage therapy.

The paper compares INPHARED database snapshots from 2021 to 2026, showing growth in phage genomes but reduced novel species diversity and persistent host bias.

The paper identifies a novel class of contractile injection system effectors in actinobacteria that enhance bacterial adaptation to competition by modulating proteomes and phenotypes.

This study identifies DUF4062 as TIR-like NADases involved in bacterial antiphage defense and eukaryotic RNA vault function through structural and biochemical analyses.

This study identifies and characterizes 29 host-modulating effectors from the mycobacteriophage NBJ that inhibit mycobacterial growth and contribute to phage defense mechanisms.

This study shows that ICP1 bacteriophage treatment effectively clears Vibrio cholerae from zebrafish larvae intestines, independent of microbiome composition.

This study identifies antiviral defense systems in haloarchaea and finds that hosts with more defense systems are less susceptible to viral infection, while receptor availability primarily determines host range.

The paper investigates the evolutionary dynamics of carotovoricin in Pectobacterium, finding that tail fiber gene exchange maintains diversity and influences community interactions.

This study analyzes phage genomes to determine the frequency and mechanism of ARG acquisition, finding that ARGs are rarely present in phages and predominantly acquired via specialized transduction.

The paper characterizes DRT10, a reverse transcriptase defense system, showing it is regulated by host exonuclease SbcB and a phage-encoded SSB-like protein.

The paper presents a structural atlas of the Pakpunavirus P7-1 phage, identifying key structural components and their roles in virion stability and genome ejection.

This study shows that phenotypic heterogeneity in Klebsiella pneumoniae affects phage resistance and cocktail efficacy, with acapsular variants being more susceptible to phages and sustaining cocktail effectiveness under selective pressure.

This study evaluates machine learning tools for predicting phage-host interactions across taxonomic levels, finding significant variability in performance, especially at finer resolutions like Family and Species.

This paper characterizes a novel Pectobacterium phage, identifies its receptor-binding proteins, and demonstrates LPS as a key receptor for phage adsorption, providing insights for phage therapy against taro soft rot.

This study characterizes a Type V BREX defense system in Haloarcula hispanica and shows that two archaeal viruses evade it via a virion protein.

This paper identifies extensive lysine acetylation in bacteriophage proteins during infection, revealing host-driven modifications that affect phage replication and infectivity.

This study identifies prophage-encoded receptor-binding proteins (RBPs) mediating capsule specificity in Klebsiella pneumoniae, revealing structural diversity and functional activity beyond classical depolymerases.

This study identifies a post-translational toxin-antitoxin mechanism in Retron-Sen3 that activates phage defense independently of ssDNA synthesis and RecBCD surveillance.

The paper identifies small molecule inhibitors of the Thoeris antiphage immune system in bacteria, showing that persistent immunity is required to prevent phage revival and population collapse.

This paper introduces a high-throughput droplet microfluidics method to quantify individual phage infection events, offering a more accurate understanding of phage-host dynamics.

This study identifies a phage-resistant sub-population of Eggerthella lenta in mice, linked to reversible genomic inversions and altered capsule production.

This study shows that phage-mediated lysis increases the growth rate of surviving bacterial cells by recycling nutrients, which partially compensates for cell loss and affects population dynamics.

This study elucidates the stepwise firing mechanism of the Algoriphagus machipongonensis extracellular contractile injection system using cryo-EM and structure-guided engineering.

This paper identifies Ltr as a key regulator of late gene expression in phage {Phi}13, showing its role in phage assembly and lysis, and reveals host factors that influence this process.

The paper identifies pre-existing anti-prophage antibodies and bacterial heteroresistance as factors contributing to phage therapy failure in a patient with chronic infection.

This paper identifies a novel bacteriophage endolysin LysPH with broad antibacterial activity and elucidates its structural basis for substrate recognition and catalytic activation.

The paper shows that phage-host predictions from MAGs are unreliable due to scaffold-level errors and proposes a validation framework to improve accuracy.