Parasitic Infections and Diagnostics

This study identifies two peptoids, TM9 and TM19, with potent anti-Cryptosporidium parvum activity that does not harm host cells, suggesting their potential as new therapeutics for cryptosporidiosis.

This study investigates how benzoxaboroles inhibit CPSF3 in Cryptosporidium parvum, identifies resistance mechanisms, and shows that AN7973 can overcome resistance.

This study shows that NOD2 stimulation with MDP reduces Cryptosporidium parvum infection in neonatal mice by increasing antimicrobial peptides and epithelial cell renewal.

The paper identifies genomic loci associated with virulence in Cryptosporidium parvum by analyzing genetic crosses and whole-genome sequencing in mice.

The paper develops a method to detect and identify minority Cryptosporidium species in mixed infections using amplicon sequencing and DADA2, demonstrating sensitivity to low-abundance variants.

This study demonstrates that oropharyngeal infection with Encephalitozoon cuniculi causes interstitial pneumonia in mice, with severity influenced by immunosuppression.

This study uses targeted CRISPR screens to identify essential genes for Cryptosporidium survival, finding Cp23 is critical for gliding motility and reinfection.

This paper identifies CpeIF4A as a novel drug target for anti-cryptosporidial therapeutics and shows that Roc-A inhibits parasite growth in vitro and in vivo.

The paper uses a CRISPR-Cas9 screen to identify host genes critical for Cryptosporidium survival, revealing a metabolic dependency on host cholesterol biosynthesis that can be targeted for treatment.

This study shows Giardia intestinalis induces a Type 2 immune response that reduces inflammation in the gut, protecting against inflammatory bowel-like diseases.

This study shows Giardia increases macrophage IL-10 production in response to LPS via MGL1, suggesting a mechanism for its anti-inflammatory effects.

The paper screens compounds in C. elegans to identify benzenesulfonamides that inhibit microsporidia spores, showing effectiveness against multiple species.

This study creates a tissue atlas of Cryptosporidium parvum infection in neonatal calves and immunocompromised mice to compare infection patterns and host-parasite interactions.

This study identifies a Cryptosporidium virulence factor that modifies host intestinal microvilli by interacting with host proteins, similar to a bacterial virulence factor.

This study uses scRNA-seq to analyze transcriptional changes in human macrophages and Encephalitozoon intestinalis parasites during infection, revealing parasite developmental dynamics and host response.

The paper uses cryo-electron tomography to reveal the in situ architecture of the polar tube invasion apparatus in microsporidian parasites.

This study identifies polycistronic mRNA expression in Cryptosporidium parvum using long-read RNA-seq and ATAC-seq, revealing a novel gene regulation mechanism in apicomplexan parasites.

Parapipe is a bioinformatics pipeline for analyzing Cryptosporidium NGS data, enabling variant calling, MOI analysis, and phylogenomic clustering to improve public health surveillance.

This paper estimates global Cryptosporidium prevalence in cattle at 25.5% and identifies C. parvum as the most common species, particularly the IIa subfamily, in diarrheic cases.

The paper presents a chromosome-level genome assembly and annotation of Cryptosporidium meleagridis, revealing 3,923 protein-coding genes and high completeness.

This study investigates the role of cAMP signaling in Giardia, focusing on PKA-mediated regulation of encystation and subcellular interactions using Split-Halo technology.

This study develops a pumpless microfluidic device for in vitro cultivation of Cryptosporidium parvum, showing extended development and infectivity of oocysts.

This study compares the in vitro efficacy and safety of four anti-cryptosporidial agents against Cryptosporidium parvum using host cells and molecular assays.

This study investigates how age-related digestive parameters affect Cryptosporidium parvum infection using an in vitro gastrointestinal model, finding differences in sporozoite release, activity, and invasiveness between adults and infants.

This study shows that Cryptosporidium infection disrupts bile acid and lipid homeostasis in the gut-liver axis, and bile supplementation reduces infection burden and restores intestinal epithelial function.

This study assesses the prevalence and removal efficiency of Cryptosporidium and Giardia in two South African wastewater treatment plants across different treatment stages.

The paper uses whole genome sequencing to identify three distinct populations of Cryptosporidium parvum, showing the emergence and spread of a European population to the USA.

This study uses CES-Seq to sequence Cryptosporidium genomes directly from clinical samples, revealing genetic diversity and enabling phylogenomic analysis and GWAS.

The paper presents a gapless telomere-to-telomere genome assembly of Cryptosporidium parvum IOWA, annotated with reference gene IDs and RNA evidence.

The paper investigates E. bieneusi prevalence in cats and dogs in Shanghai, finding genotype D, a zoonotic pathogen, in 9.5% of samples with higher rates in stray animals.