Legionella and Acanthamoeba research

This study identifies optimal biotic and abiotic conditions for long-term co-culture of Pseudomonas aeruginosa and Acanthamoeba castellanii, achieving high viability for both species over 14 days.

This paper uses human cerebral organoids to model Balamuthia mandrillaris infection, revealing host responses, biomarkers, and the therapeutic potential of nitroxoline.

This study determines the crystal structure of Legionella collagen-like protein and reveals its unique binding mechanism for sulphated glycosaminoglycans.

This study identifies opposing roles of tissue-resident alveolar macrophages and infiltrating neutrophils/monocyte-derived cells in controlling Legionella longbeachae infection via the IL-18R/IFN-γ/ROS pathway.

This study shows that Legionella pneumophila replication depends on host membrane acyl chain composition, with palmitoleic acid promoting membrane disorder and enhancing bacterial growth.

This paper develops a machine learning method to accurately attribute Legionella pneumophila outbreaks to environmental sources using genomic data, outperforming traditional phylogenetic approaches.

This study determines the structure of LceB, a core effector from Legionella pneumophila, revealing its conformational flexibility, self-association, and potential host protein-binding surface.

The paper investigates predation-resistant Pseudomonas strains and their interactions with Dictyostelium discoideum, finding that some cause extracellular infections and suppress predation of other bacteria.

This study identifies how Legionella pneumophila effectors modulate Rab10 dynamics through ubiquitin signaling, phosphoribosylation, and crosslinking to control bacterial vacuole function.

This study identifies MavH as a Legionella effector that promotes membrane-dependent actin polymerization by interacting with PI(3)P and host actin capping proteins.

The paper presents chromosome-scale genome assemblies of Acanthamoeba castellanii and shows infection-induced chromatin reorganization during Legionella pneumophila infection.

The paper reports the structural determination of 19 N. fowleri proteins, identifying potential drug targets with structural differences from human homologs.

The paper identifies over 16,000 secreted proteins in Legionella species, revealing their ability to acquire eukaryotic-like genes for intracellular replication in human cells.

Genome sequencing of Legionella pneumophila isolates from a Sydney outbreak identified an emerging ST211 clone linked to environmental sources and a unique T4ASS element.