RNA regulation and disease

This study shows that guided A>I snRNAs improve RNA base conversion efficiency and reduce off-target effects compared to ADAR-recruiting circular RNAs in human cells.

This paper introduces modulADAR, a modular RNA sensor that enables detection of short RNA subsequences by leveraging ADARs' editing capabilities, overcoming limitations of previous sequence-dependent sensors.

This study shows that ZYS-1 is not a selective inhibitor of ADAR1, as it does not reduce A-to-I editing or affect ADAR1-dependent cell line survival.

The paper links ADAR1-mediated RNA editing in smooth muscle cells to regulation of MDA5 activation, showing its role in atherosclerosis progression and vascular calcification.

This study investigates the effects of Adar in Drosophila melanogaster, showing that overexpression of certain Adar isoforms leads to larval lethality and aberrant immune responses, with partial rescue through epigenetic modulation.

The paper introduces CellREADR, an RNA sensor-effector technology for targeting and manipulating specific human cortical neuron types, validated through histological, electrophysiological, and transcriptomic methods.

The paper analyzes MPXV RNA-seq data to determine if APOBEC3 enzymes edit viral RNA, concluding that observed mutations result from DNA-level mutagenesis, not RNA editing.

This paper develops a method for targeted RNA editing using a covalent adenosine deaminase-antisense oligo conjugate, achieving efficient and specific A-to-I editing with reduced off-target effects.

This study identifies DDX39B as a global repressor of A-to-I RNA editing using CRISPR screens and shows its potential as a therapeutic target for anti-HDV therapy.

The paper identifies ADAR1 as a host factor that restricts poliovirus infection by editing viral RNA to disrupt translation initiation and introduce a coding error.

The paper develops ADAR-mediated fluorescent RNA sensors to specifically track C3+ reactive astrocyte subpopulations in iPSC-derived models.

This study shows APOBEC3A deaminates tRNAs in vitro and identifies editing sites in tumor tissues, suggesting a role in mistranslation and cancer.

The study shows that Z domain mutations in ADAR1p150 slightly reduce A-to-I editing, likely due to mislocalization rather than editing specificity loss, and suggests phenotypes may stem from ZBP1 inhibition.

This paper develops a novel U7 snRNA scaffold that significantly enhances ADAR-mediated RNA base editing in vitro and in vivo, achieving up to 76% editing efficiency in mouse models.

This paper describes a mouse model with a human ADAR G1007R mutation that causes neuroinflammation and shows that deleting MDA5 can reverse brain inflammation.

The paper shows that astrocytic RNA editing via ADAR1 modulates immune responses to alpha-synuclein, contributing to neuroinflammation in Parkinson's disease.

This paper uses a deaminase-based RNA recording method to profile protein-RNA interactions by introducing nucleotide edits detectable via DNA sequencing, revealing conserved sequence requirements across different contexts.

This study introduces synthetic PPR editors for precise RNA base editing in plant organelles, showing efficient editing with minimal off-target effects.

This study shows ADAR1 p150 prevents HSV-1 from activating PKR/eIF2alpha, allowing efficient viral replication.

The paper introduces LIDAR, a post-transcriptional synthetic receptor platform using RNA editing to sense ligands and produce functional outputs, enabling complex multicellular behavior programming.

The paper analyzes RNA editing in myeloid cells under inflammatory stimulation, finding that C-to-U editing has a larger effect on proteome recoding compared to A-to-I editing.

The paper challenges the validation of ZYS-1 as an ADAR1 inhibitor, showing it lacks inhibitory activity in validated assays.

The paper develops dsRNAscan to predict dsRNAs in the human genome, identifies neuronal enrichment and intermolecular interactions, and provides a resource for dsRNA annotations.

The paper shows that ADAR1 haploinsufficiency combined with viral RdRp dsRNA synthesis causes dysregulated RNA editing and multi-system interferonopathy.

This study characterizes ADAR1's RNA editing mechanism through biochemical profiling and structural analysis, revealing sequence and duplex length dependence, disease mutation impacts, and the role of RNA-binding domain 3.

The paper introduces CellREADR, an RNA-based technology for targeting and manipulating animal cells by coupling RNA sensing with effector protein translation, and provides a detailed experimental protocol for its implementation.

This study shows that ADAR1 dimerization affects site-specific RNA editing, with dimer disruption altering editing efficiency at specific sites without abolishing overall activity.

This paper introduces T2, a method using RNA editing to estimate transcriptional age and identify transient transcriptional programs in human cells.

TSniffer identifies RNA editing sites and quantifies editing activity in RNA-seq data, revealing widespread ADAR-mediated editing in mammals.