Rationally Designed Pooled CRISPRi-seq Uncovers an Inhibitor of Bacterial Peptidyl-tRNA Hydrolase
A. S. M. Z. Rahman, E. A. Syroegin, J. Novomisky Nechcoff, A. Devarajan, Y. S. Polikanov, S. T. Cardona
The paper introduces CIMPLE, a rationally designed CRISPRi pooled library, to uncover antibiotic targets by improving genomic coverage of essential genes during drug exposure.
bioRxiv · May 2, 2024Microbiology