D. M. Margolis

The histone methyltransferase SETD2 regulates HIV expression and latency through a post-transcriptional mechanism.

This study shows that SETD2 inhibition reduces HIV gene expression and latency by altering viral RNA splicing, not transcription.

Machine learning approaches identify immunologic signatures of total and intact HIV DNA during long-term antiretroviral therapy.

The paper uses machine learning to analyze HIV reservoir and immunology data to identify immune signatures associated with total and intact HIV DNA levels during ART.

A novel high throughput microwell outgrowth assay for HIV infected cells

The paper introduces a high-throughput microwell outgrowth assay to quantify and characterize HIV-infected cells with high sensitivity and precision.

A truncated HIV Tat demonstrates potent and specific latency reversal activity

The paper demonstrates that a truncated HIV Tat protein and mRNA can reactivate HIV-1 in latently infected T-cells as effectively as Phorbol 12-Myristate 13-Acetate without T-cell activation or significant transcriptome changes.

Impact of cannabis use on immune cell populations and the viral reservoir in people with HIV on suppressive antiretroviral therapy.

This study examines how cannabis use affects immune cell populations and the HIV reservoir in HIV-positive individuals on antiretroviral therapy, finding reduced T cell activation and potential reservoir size reduction in some subgroups.

Integrated single-cell multiomic analysis of HIV latency reversal reveals novel regulators of viral reactivation.

The paper uses single-cell RNA-seq and ATAC-seq to analyze HIV latency reversal, identifying GATA3 as a novel regulator and demonstrating a machine learning model's predictive accuracy.

Large-scale allosteric switch in the 7SK RNA regulates transcription in response to growth and stress

The paper introduces DANCE-MaP, a chemical probing strategy to characterize RNA structure and dynamics, revealing 7SK RNA's structural switch regulates transcription in response to growth and stress.

Epigenomic characterization of latent HIV infection identifies latency regulating transcription factors.

This study identifies transcription factors regulating HIV latency through epigenomic analysis of latently infected cells.

Sequence evaluation and comparative analysis of novel assays for intact proviral HIV-1 DNA

This study compares two novel assays, IPDA and Q4PCR, for measuring intact HIV proviral DNA in ART-suppressed individuals, finding significant differences in their frequency estimates.

The Replication-Competent HIV-1 Latent Reservoir is Primarily Established Near the Time of Therapy Initiation

The paper shows that most of the long-lived HIV-1 latent reservoir forms near the time of antiretroviral therapy initiation, not continuously during untreated infection.

Single cell analysis of quiescent HIV infection reveals host transcriptional profiles that regulate proviral latency

This study uses single-cell methods to analyze HIV latency, finding that host transcriptional profiles correlate with proviral activity levels in infected cells.