J. A. Doudna

This study shows that CRISPR-Cas12a activates non-specific single-stranded DNA cleavage upon guide RNA-dependent DNA binding, distinguishing it from CRISPR-Cas9.

This study identifies a mechanism by which CRISPR-Cas9 variants discriminate target mismatches and introduces a hyper-accurate variant with improved specificity.

The paper investigates translational changes during human neuronal differentiation and identifies mTOR complex 1 signaling and 3' UTR regulation as key factors.

This paper describes a thermostable Cas9 variant from Geobacillus stearothermophilus that functions at higher temperatures and has increased stability in human plasma, expanding CRISPR-Cas9 applications.

This study shows that AcrIIA4 inhibits Cas9 by binding to Cas9-sgRNA complexes, reducing off-target edits while allowing on-target gene editing in human cells.

This study identifies a conformational checkpoint in CRISPR-Cas9 that regulates DNA cleavage by requiring divalent cations and preventing cleavage at off-target sites with sequence mismatches.