J. C. Fromme

Sec7 regulatory domains scaffold autoinhibited and active conformations

This study determines the cryoEM structure of autoinhibited Sec7 and proposes an active conformation model based on functional and structural analyses.

The Arf-GAP Age2 localizes to the late-Golgi via a conserved amphipathic helix

The paper identifies a conserved amphipathic helix in Age2 that mediates its Golgi localization and reports a probe for sensing active Arf1 in living cells.

Activity-based directed evolution of a membrane editor in mammalian cells

This paper develops a membrane editor using engineered phospholipase D variants to modify phospholipid composition in cells and in vitro.

Structural basis for activation of Arf1 at the Golgi complex

This study presents cryoEM structures of Gea2, revealing its conformational changes during Arf1 activation and how it binds to the Golgi membrane.

Structure of a TRAPPII-Rab11 activation intermediate reveals GTPase substrate selection mechanisms

The paper presents cryoEM structures of the TRAPPII complex from budding yeast, revealing mechanisms of Rab11 specificity and nucleotide exchange.

Structures of core eukaryotic protein complexes

The paper uses coevolution analysis and deep learning to model core eukaryotic protein complexes in yeast, identifying 104 new assemblies and structurally characterizing 608 previously unknown complexes.

Surpassing the physical Nyquist limit to produce super-resolution cryo-EM reconstructions

This paper demonstrates super-resolution cryo-EM reconstructions that surpass the physical Nyquist limit using a K3 direct electron detector and shows the benefits of lower magnifications for increased particle numbers.

Structure and mechanism of TRAPPIII-mediated Rab1 activation

This study determines the cryo-EM structure of the yeast TRAPPIII complex bound to Rab1/Ypt1, revealing that Trs85 anchors the complex to membranes via an amphipathic helix to activate Rab1.

Structural basis for the initiation of COPII vesicle biogenesis

The paper reports the structure of the Sar1-Sec12 GEF complex, revealing how Sec12 activates Sar1 for COPII vesicle formation.