J. Shanklin

Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress

Biotic and heat stress strongly alter the lipidome and proteome of Arabidopsis leaves, including lipid droplet proteome composition.

Genomics of turions from the Greater Duckweed reveal its pathways for dormancy and reemergence strategy

This study analyzes the transcriptome and epigenome of turions from Greater Duckweed to identify molecular pathways involved in dormancy and reemergence.

AlphaFold Protein Structure Database for Sequence-Independent Molecular Replacement

The paper tests if AlphaFold-predicted protein structures can be used for sequence-independent molecular replacement in crystallography, successfully determining two unknown contaminant structures.

Structural Basis for SARS-CoV-2 Envelope Protein in Recognition of Human Cell Junction Protein PALS1

The paper determines the structure of the SARS-CoV-2 E protein-PALS1 complex using cryo-EM, revealing a hydrophobic interaction that explains viral spread and suggesting therapeutic targets.

Structural Basis for Ca2+-Dependent Activation of a Plant Metacaspase

This study determines the crystal structure of AtMC4, revealing how Ca2+ activates plant metacaspase through linker domain cleavage and substrate access.

An Expanded Role for WRINKLED1 Metabolic Control Based on Combined Phylogenetic and Biochemical Analyses

The paper identifies conserved WRI1 binding sites in central metabolism and lipid biosynthesis using comparative genomics and in vitro DNA binding assays.

BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins, inhibitors of ACCase, are regulated by WRINKLED1

WRI1 regulates genes encoding fatty acid synthesis and BADCs, which inhibit FA synthesis, creating a coordinated lipid homeostasis mechanism.