Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron
H. A. Arjes, J. Sun, H. Liu…·A. L. Shiver
The paper constructs an ordered mutant collection of Bacteroides thetaiotaomicron using transposon-insertion and barcode sequencing, identifies factors limiting coverage, and demonstrates phenotypic screening for growth defects and cell shape changes.