L. Pollack

Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron

This study uses mix-and-inject serial crystallography to resolve the catalytic mechanism of DJ-1, showing it catalyzes nearly two turnovers and reveals allosteric communication during glyoxalase activity.

Defining the conformational states that enable transglutaminase 2 to promote cancer cell survival versus cell death

This paper investigates the conformational states of TG2 and shows how an inhibitor stabilizes an open conformation that is cytotoxic to cancer cells.

Length-dependent Intramolecular Coil-to-Globule Transition in Poly(ADP-ribose) Induced by Cations

This study investigates how the length of poly(ADP-ribose) (PAR) affects its structural transitions in the presence of Mg2+ ions, revealing length-dependent compaction and bundle formation.

RNA structures and dynamics with A resolution revealed by x-ray free electron lasers

This paper reveals RNA structures and dynamics at near-atomic resolution using x-ray free electron lasers, showing how RNA folds through intermediate states and forms triplexes.

RNA triplex structures revealed by WAXS-driven MD simulations

This paper uses WAXS-driven MD simulations to reveal RNA triplex structures that are difficult to characterize by crystallography.

Direct Observation of the Mechanism of Antibiotic Resistance by Mix-and-Inject at the European XFEL

The paper directly observes fast ligand binding in a biomedically relevant enzyme using MHz X-ray pulses at the European XFEL.

Enzyme Intermediates Captured "on-the-fly" by Mix-and-Inject Serial Crystallography

This paper demonstrates a method to capture enzyme reactions in real-time using mix-and-inject serial crystallography, revealing antibiotic cleavage and kinetic transitions in Mycobacterium tuberculosis β-lactamase.