N. Smith

Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron

This study uses mix-and-inject serial crystallography to resolve the catalytic mechanism of DJ-1, showing it catalyzes nearly two turnovers and reveals allosteric communication during glyoxalase activity.

Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators.

This study uses machine learning to predict and engineer improved fluorescent calcium indicators, identifying variants with faster kinetics and larger responses.

Far-red and sensitive sensor for monitoring real-time H2O2 dynamics with subcellular resolution and inmulti-parametric imaging applications.

This paper introduces a new fluorescent sensor, oROS-HT, for real-time H2O2 monitoring with subcellular resolution and multi-parametric imaging capabilities.

Structure-guided engineering of a fast genetically encoded sensor for real-time H2O2 monitoring.

This paper develops a fast, sensitive genetically encoded H2O2 sensor (oROS-G) and demonstrates its use in monitoring H2O2 dynamics in various cell types and models.

X-ray-driven chemistry and conformational heterogeneity in atomic resolution crystal structures of bacterial dihydrofolate reductases

This study shows X-rays can drive hydride transfer in bacterial DHFRs, revealing conformational changes and catalytic dynamics through high-resolution crystal structures.

Multiple Intermediates in the Detergent-Induced Fusion of Lipid Vesicles

This study identifies multiple intermediates in detergent-induced vesicle fusion using FRET and single-vesicle analysis, revealing a multi-step mechanism involving permeabilization, docking, hemi-fusion, and lipid mixing.

A benzoxazolylurea inhibits VraS and enhances antimicrobials against vancomycin intermediate-resistant Staphylococcus aureus

Post-glacial recolonization and multiple scales of secondary contact contribute to contemporary Atlantic salmon (Salmo salar) genomic variation in North America.

This study uses genomic data from Atlantic salmon to show that post-glacial recolonization and secondary contact at multiple scales shaped contemporary genetic diversity in North America.

Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography

The paper uses XFEL crystallography to observe conformational changes in an enzyme during catalysis, revealing ionization-linked dynamics that control catalytic activity.

DNA Repair Function Scores for 2172 Variants in the BRCA1 Amino-Terminus

The paper presents functional scores for 2172 BRCA1 variants using a multiplexed HDR assay, showing 93% sensitivity and 100% specificity in classifying pathogenic and benign variants.

Disentangling the effects of eutrophication and natural variability on macrobenthic communities across French coastal lagoons

This study examines how eutrophication and natural variability affect macrobenthic communities in French coastal lagoons using environmental and biological data.

Zmiz1 is required for mature β-cell function and mass expansion upon high fat feeding

This study shows that Zmiz1 is essential for β-cell function and mass expansion in response to high fat feeding, linking its loss to diabetes.

β-cell responses to high fat feeding: A role and mechanism for redox sensing by SENP1

This study identifies SENP1's role in β-cell redox sensing during high-fat feeding, showing its requirement for enhanced exocytosis and glucose intolerance.

Lin28b specifies an innate-like lineage of CD8+ T cells in early life

The paper shows Lin28b programs neonatal CD8+ T cells to have innate defense capabilities through transcriptional and epigenetic changes.

Impacts of the COVID-19 Pandemic on a Human Research Islet Program

This study examines how the COVID-19 pandemic affected islet processing and distribution at a human research islet facility, finding reduced organ processing but maintained islet quality, though recipient feedback was poorer due to longer transit times.

Nanotrap Particles Improve Nanopore Sequencing of SARS-CoV-2 and Other Respiratory Viruses

The paper introduces a magnetic hydrogel particle method to improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses from diagnostic samples.

Targeting the chemokine receptor CXCR4 with histamine analogue to reduce inflammation in juvenile arthritis: a proof of concept for COVID-19 therapeutic approach

This study shows that targeting CXCR4 with clobenpropit reduces inflammation in juvenile arthritis and COVID-19 by lowering cytokine levels and immune cell activity.

GLOBAL PATTERNS OF PLUMAGE COLOUR EVOLUTION IN ISLAND-LIVING PASSERIFORM BIRDS

The paper examines plumage color evolution in island-living passeriform birds, finding sex-specific differences and ecological influences on color variation across families.

Altered interactions between circulating and tissue-resident CD8 T cells with the colonic mucosa define colitis associated with immune checkpoint inhibitors

This study identifies altered interactions between circulating and tissue-resident CD8 T cells with the colonic mucosa in immune checkpoint inhibitor-associated colitis using single-cell RNA sequencing and other multi-omics approaches.

Heterogenous impairment of α-cell function in type 2 diabetes is linked to cell maturation state

This study identifies heterogeneous α-cell dysfunction in type 2 diabetes linked to impaired maturation and electrophysiological changes.

A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

A bispecific nanobody neutralizes SARS-CoV-2 and its variant B.1.351 by inducing spike trimer-dimers that prevent ACE2 binding.

A monocyte/dendritic cell molecular signature of SARS-CoV2-related multisystem inflammatory syndrome in children (MIS-C) with severe myocarditis

The paper identifies a monocyte/dendritic cell gene signature in severe MIS-C with myocarditis, linked to NF-κB activity and cytokine signaling.

Tuberculosis alters immune-metabolic pathways resulting in perturbed IL-1 responses

The paper examines how tuberculosis alters immune-metabolic pathways, leading to changes in IL-1 responses in TB patients compared to latent infection and post-treatment.

β-cell SENP1 facilitates responsiveness to incretins and limits oral glucose intolerance in high fat fed mice

This study shows that β-cell SENP1 improves insulin secretion in response to incretins and reduces oral glucose intolerance in high-fat-fed mice.

SARS-CoV-2 infection damages airway motile cilia and impairs mucociliary clearance

This study shows SARS-CoV-2 infection damages airway motile cilia and impairs mucociliary clearance, potentially facilitating viral spread to the deeper lung.

Pathogenic mutations in LRRK2 sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia

This study shows that LRRK2 mutations sequester Rab8a into lysosomes and disrupt transferrin-mediated iron uptake in microglia, linking LRRK2 dysfunction to iron dysregulation in Parkinson's disease.

Mutations in the SPTLC1 gene are a cause of juvenile amyotrophic lateral sclerosis that may be amenable to serine supplementation

The paper identifies de-novo SPTLC1 mutations in juvenile ALS patients and suggests serine supplementation may alleviate symptoms.

An alternative AUG codon in segment 5 of the 2009 pandemic influenza A virus is a swine-derived virulence motif

The paper identifies an alternative AUG codon in the 2009 pandemic influenza A virus as a virulence factor contributing to pathogenicity in swine and humans.

Treatment of mice with IL2-complex enhances inflammasome-driven IFN-γ production and prevents lethal toxoplasmosis

This study shows that IL2C treatment expands CD8+ T cells, DN T cells, and NK cells, enhancing IFN-γ production and preventing lethal toxoplasmosis in mice.

Thermal acclimation of leaf respiration consistent with optimal plant function

The paper examines how leaf respiration responds to temperature changes and its implications for carbon-climate feedbacks.