A. Leung

PARP1 condensates differentially partition DNA repair proteins and enhance DNA ligation

This study shows PARP1 condensates partition DNA repair proteins and enhance DNA ligation through PARylation-dependent mechanisms.

Length-dependent Intramolecular Coil-to-Globule Transition in Poly(ADP-ribose) Induced by Cations

This study investigates how the length of poly(ADP-ribose) (PAR) affects its structural transitions in the presence of Mg2+ ions, revealing length-dependent compaction and bundle formation.

PARP10 is Critical for Stress Granule Initiation

This study identifies PARP10 as essential for stress granule initiation, showing it regulates assembly kinetics and modifies G3BP1 to influence SG composition.

Discovery of compounds that inhibit SARS-CoV-2 Mac1-ADP-ribose binding by high-throughput screening

This study identifies 5 compounds that inhibit SARS-CoV-2 Mac1-ADP-ribose binding through high-throughput screening, with IC50 values under 100 µM.

Design, Synthesis and Evaluation of Inhibitors of the SARS-CoV-2 nsp3 Macrodomain

This study designs and synthesizes inhibitors targeting the SARS-CoV-2 nsp3 macrodomain, identifying compound 15c as a low-micromolar inhibitor with structural and thermal evidence of binding.

Translational readthrough as a potential therapeutic for AIPL1-associated Leber Congenital Amaurosis in a patient-derived iPSC-retinal organoid model

This study uses patient-derived iPSC-retinal organoids to investigate translational readthrough as a therapy for LCA4 caused by AIPL1 mutations.

Crystal structures and functional analysis of the ZnF5-WWE1-WWE2 region of PARP13/ZAP define a new mode of engaging poly(ADP-ribose)

The paper presents crystal structures of the ZnF5-WWE1-WWE2 region of PARP13 and shows it binds poly(ADP-ribose) through WWE2, revealing a new mode of recognition.

High-throughput Activity Assay for Screening Inhibitors of the SARS-CoV-2 Mac1 Macrodomain

The paper develops a high-throughput assay for screening inhibitors of the SARS-CoV-2 Mac1 macrodomain and identifies dasatinib and dihydralazine as inhibitors with selectivity over human MacroD2.

The DarTG toxin-antitoxin system provides phage defense by ADP-ribosylating viral DNA

This study identifies DarTG toxin-antitoxin systems in E. coli that defend against phage by ADP-ribosylating viral DNA, with phages evolving resistance through DNA polymerase or anti-DarT factors.

Exposure route, sex, and age influence disease outcome in a golden Syrian hamster model of SARS-CoV-2 infection

This study investigates how exposure route, sex, and age affect SARS-CoV-2 pathogenesis in golden Syrian hamsters, finding significant differences in disease outcomes.

Distinct SARS-CoV-2 antibody reactivity patterns elicited by natural infection and mRNA Vaccination

The paper compares antibody reactivity patterns between natural SARS-CoV-2 infection and mRNA vaccination, finding that vaccination induces stronger and broader antibody responses, including cross-reactivity against SARS antigens.

Discovery and biosynthesis of clostyrylpyrones from the obligate anaerobe Clostridium roseum

The paper discovers a new family of natural products, clostyrylpyrones, from Clostridium roseum and proposes their polyketide synthase-based biosynthesis mechanism.

North American deer mice are susceptible to SARS-CoV-2

This study shows North American deer mice can be infected and transmit SARS-CoV-2, suggesting they may serve as a potential reservoir for the virus.