Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS
T. O. T. Lambert, M. Gramlich, L. S. Stutzke, L. Smith, D. Deng, P. D. Kaiser, U. Rothbauer, J. L. Benesch, C. Wagner, M. Koenig, P. Pompach, P. Novak, A. Zeck, K. D. Rand
The paper develops a PNGase Rc column for online deglycosylation during HDX-MS, enabling efficient analysis of glycoproteins and mapping of mAb binding epitopes.
bioRxiv · Jul 28, 2023Biochemistry