U. Rothbauer

Long-term single molecule localization microscopy uncovers dynamic co-assembly of Lrp6 and Ror2 into Wnt-signalosomes

This study uses single-molecule tracking to show that Lrp6 and Ror2 co-assemble into hybrid Wnt signalosomes dynamically.

Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1

This study develops nanobodies to monitor Drp1, a mitochondrial fission factor, using proteomics, microscopy, and live-cell imaging techniques to study its dynamics in disease models.

Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS

The paper develops a PNGase Rc column for online deglycosylation during HDX-MS, enabling efficient analysis of glycoproteins and mapping of mAb binding epitopes.

Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells

This study develops human SIRPα nanobodies for both functional modulation of myeloid cells and in vivo imaging of tumor infiltration.

A novel PNGase Rc for improved protein N-deglycosylation in bioanalytics and HDX-MS epitope mapping under challenging conditions

Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors

This study develops recombinant nanobody-fused biosensors to monitor extracellular K+, pH, and glucose in real time, demonstrating their utility in cancer and neuronal cells.

A nanobody-based toolset to monitor and modify the mitochondrial GTPase Miro1

This study develops Miro1-specific nanobodies as tools for monitoring and modifying Miro1 function in cells, with applications in imaging and targeted degradation.

A broadly neutralizing biparatopic Nanobody protects mice from lethal challenge with SARS-CoV-2 variants of concern

This study identifies a biparatopic nanobody that protects mice from lethal SARS-CoV-2 infection and reduces viral load and inflammation.

Single-domain antibodies for targeting, detection and in vivo imaging of human CD4+ cells

This study develops single-domain antibodies to target and image human CD4+ cells in vivo, demonstrating their utility in immunotherapy monitoring.

NeutrobodyPlex - Nanobodies to monitor a SARS-CoV-2 neutralizing immune response

The paper develops NeutrobodyPlex, a multiplex assay using bivalent nanobodies to analyze neutralizing antibodies against SARS-CoV-2 in patient sera.

A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies

This paper introduces a PepTag/PepCB system for real-time visualization and quantification of antigens in live cells using fluorescently labeled chromobodies.