M. S. Siegrist

The paper develops CHAMP, a method to measure cytosolic accumulation of azide-tagged molecules in Gram-negative bacteria, enabling high-throughput screening of over 1,000 compounds.

The paper develops a novel assay to measure small molecule permeation across the mycomembrane of mycobacteria, revealing variability in permeability profiles between species.

The paper identifies tuberculostearic acid (TBSA) as a key lipid controlled by the gene cfa, which regulates membrane compartmentalization in Mycobacterium smegmatis.

This study shows that mycobacteria rapidly modify phosphatidylinositol mannosides (PIMs) by adding an inositol acyl chain in response to membrane fluidization, enhancing resistance to membrane stress.

The paper develops BaCAPA, a method to measure small molecule accumulation in specific bacterial compartments, showing its utility for both model and pathogenic bacteria.

The paper synthesizes and demonstrates the metabolic processing of a selenium-based bioisostere of m-DAP in live bacteria, showing its incorporation into the peptidoglycan layer.

This study investigates the role of RodA in mycobacterial cell wall synthesis under damage, showing its non-redundant function in spatially flexible peptidoglycan assembly and stress response.

The paper shows that in Mycobacterium smegmatis, peptidoglycan precursors are synthesized in distinct plasma membrane domains that are separate from sites of cell wall assembly, and this partitioning is essential for ordered synthesis.

This study shows that trehalose recycling reduces energy costs for mycomembrane remodeling in nutrient-starved mycobacteria, enhancing survival under stress.

The paper investigates peptidoglycan precursor synthesis in mycobacteria, showing that sidewall synthesis responds to cell wall damage while polar synthesis is linked to elongation.

This study shows DivIVA concentrates cell wall synthesis in mycobacteria to initiate and stabilize polar growth, supporting the idea that rod shape is a hardwired bacterial trait.