BiochemistrybioRxiv
D/E-rich peptides are less suitable than D/E-deficient peptides for identification by negative-ion HCD due to scarce production of sequencing ions from multiply charged precursors
M.-Q. Zuo, R.-X. Sun, M.-Q. Dong
The paper shows D/E-rich peptides produce fewer sequencing ions in negative-ion HCD MS, making them harder to identify, and suggests using proteases that generate D/E-deficient peptides for better results.
DOI 10.1016/j.ijms.2022.116975