T. Fleming

Streamlined generation of CRISPR/Cas9-mediated single-cell knockout clones in murine cell lines.

This paper describes a method to efficiently generate and identify CRISPR/Cas9-mediated single-cell knockout clones in murine cell lines within 3-4 weeks.

Deep metabolic profiling assessment of tissue extraction protocols for three model organisms

The paper evaluates extraction protocols for metabolomic profiling in three model organisms, finding a protocol that balances reproducibility and metabolite detection across tissues.

Induction of mitochondrial heat shock proteins and mitochondrial biogenesis in endothelial cells upon acute methylglyoxal stress: Evidence for hormetic autofeedback

This study shows that knocking out HSPA1A/HSPA1B in mouse endothelial cells leads to increased mitochondrial biogenesis and chaperone expression under methylglyoxal stress.

Methylglyoxal Induces Endothelial Dysfunction via Stunning

This study shows that methylglyoxal induces reversible endothelial dysfunction through a non-proliferative state and cellular stunning, mediated by MG-H1 turnover.

SMPD3 suppresses IDH mutant tumor growth via dual autocrine-paracrine roles

This study shows SMPD3 suppresses IDH mutant glioma growth through autocrine and paracrine mechanisms, with higher SMPD3 linked to longer survival in patients.