MicrobiologybioRxiv
A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi
C. N. Takacs, M. Scott, Y. Chang…·C. Jacobs-Wagner
This paper develops a CRISPR interference platform for efficient gene downregulation in Borrelia burgdorferi, demonstrating >95% repression efficiency for multiple genes and revealing roles in motility and cell division.
DOI 10.1128/aem.02519-20