Gaudenz M. Danuser

The paper introduces a zebrafish model and computational framework to systematically analyze cancer metastasis patterns across organs, revealing heterogeneity and spatial preferences in dissemination.

The paper introduces GTPase biosensors that report activity without blocking ligand interactions, revealing feedback mechanisms in Rac1 regulation.

The paper introduces v-CyCIF, a method for 3D spatial profiling of human FFPE tissue, enabling detailed analysis of tumor, stroma, and immune structures.

The paper develops a FRET biosensor to visualize RhoG activity in live cells and shows its spatial relationship with Rac1 during protrusion.

This study identifies ARHGAP20 as a regulator of cell migration by modulating Rap1A-RhoA signaling coordination through vesicular trafficking.

This study uses optogenetics and biosensor imaging to show RhoG activates Rac1 and Cdc42 in cell protrusion, with RhoG's effects on protrusion partially mediated by Rac1 and some independent of it.

This study shows that branched actin-driven membrane protrusions (BAMPs) enhance oncogenic KRAS signaling by amplifying upstream regulators and promoting cell proliferation through merlin inactivation.

The paper reconstructs a mechanistic model of actin dynamics at the leading edge of mesenchymal cells using multiplex microscopy data and regression analysis.

This paper shows that bleb-induced septin signaling supports survival of metastatic melanoma cells in vivo, and disrupting this pathway reduces metastasis.

SPACEMAP is a machine learning framework for multiplex imaging analysis that resolves phenotyping discordance by integrating multiple classification methods.

The paper introduces u-Segment3D, a tool that converts 2D cell segmentations into 3D consensus segmentations without training data, demonstrating effectiveness across diverse datasets.

This study shows phosphoinositide lipids have distinct roles at filopodial tips and bases, affecting actin dynamics and filopodial movement in Xenopus retinal ganglion cells.

This paper introduces 3D CyCIF, a method for high-plex 3D profiling of cell states and immune niches in human tumors, revealing detailed spatial interactions and cytokine niches previously unattainable in thin sections.

This paper develops an imaging-based assay using zebrafish larvae to assess organotropism of cancer xenografts and identifies EWSR1::FLI1 and SOX6 as plasticity factors enhancing metastatic adaptability.

The paper maps the human ER subdomain proteome, identifies CLMN as an ER-actin tether at focal adhesions, and shows it promotes cell migration by regulating actin dynamics and calcium signaling.

Delta-Marches uses generative AI to simulate morphological changes between disease classes, identifying subcellular features that drive disease progression in histopathological images.

The paper introduces a self-driving, multi-resolution light-sheet microscope to simultaneously observe sub-cellular dynamics and whole organisms over extended periods, applying it to study development, cancer, and immune interactions in zebrafish.

DynamicNeuronTracker enables accurate tracking of neurons in deforming 3D calcium imaging data and reveals combinatorial response profiles of pheromone-sensing neurons to bile acids and sulfated steroids.

This study identifies Caveolin-1 as a context-dependent regulator of survival signaling in Ewing Sarcoma, revealing a reversible CD99High state with enhanced survival.

This study identifies fascin-1's role in promoting anchorage-independent proliferation through a novel signaling complex called PASS, which drives Rac1/PAK/JNK signaling in cancer cells.

This study identifies RhoA activation as a driver of glucose uptake and proliferation in MAPK inhibitor-resistant melanoma cells through ROCK-mediated IRS1 and PI3K pathway activation.

The paper introduces u-signal3D, a framework for analyzing molecular organization on cell surfaces in a morphology-invariant way, validated with synthetic and real data.

The paper introduces u-Unwrap3D to map 3D cell surfaces and analyze interactions between geometry and molecular signals, revealing new dynamics in immune and tumor cell behaviors.

This paper develops a nonlinear image registration method for live cell movies to analyze spatiotemporal dynamics of diffuse molecular processes, revealing organized Profilin dynamics linked to actin organization.