Felix Zhou

The paper introduces v-CyCIF, a method for 3D spatial profiling of human FFPE tissue, enabling detailed analysis of tumor, stroma, and immune structures.

The paper develops SPOT, a tool for analyzing 3D morpho-dynamics from 2D live-cell imaging, and applies it to organoids to identify phenotype-genotype-function relationships.

This study maps how distinct oncogenic drivers induce unique multiscale architectural changes in liver tissue, revealing mutation-specific remodeling across cellular and mitochondrial structures.

This study shows that branched actin-driven membrane protrusions (BAMPs) enhance oncogenic KRAS signaling by amplifying upstream regulators and promoting cell proliferation through merlin inactivation.

This paper introduces Cy-ExM, a high-plex imaging strategy that enables 3D super-resolution mapping of cellular ultrastructure in mammalian cells.

The paper develops a universal imaging phenome using SAM features and SPOT tool, showing it outperforms deep learning models in capturing dynamic cell behaviors.

The paper introduces u-Segment3D, a tool that converts 2D cell segmentations into 3D consensus segmentations without training data, demonstrating effectiveness across diverse datasets.

The paper introduces miOPM, a reconfigurable LSFM platform that enables high-resolution imaging across multiple spatial scales and refractive indices.

This paper introduces 3D CyCIF, a method for high-plex 3D profiling of cell states and immune niches in human tumors, revealing detailed spatial interactions and cytokine niches previously unattainable in thin sections.

The paper introduces a self-driving, multi-resolution light-sheet microscope to simultaneously observe sub-cellular dynamics and whole organisms over extended periods, applying it to study development, cancer, and immune interactions in zebrafish.

The paper investigates the role of calcium signaling in anterior-posterior axis formation in the mouse embryo, finding that SERCA inhibition disrupts DVE migration and axial patterning.

The paper characterizes the transcriptional dynamics of the mouse Anterior Visceral Enderm (AVE) during migration and identifies Ephrin- and Semaphorin-signalling as critical for its movement.

This study identifies fascin-1's role in promoting anchorage-independent proliferation through a novel signaling complex called PASS, which drives Rac1/PAK/JNK signaling in cancer cells.

The paper introduces a mesoscopic oblique plane microscope for whole-organism imaging with high speed and subcellular resolution, demonstrating its application in zebrafish vasculature and blood flow analysis.

The paper introduces u-Unwrap3D to map 3D cell surfaces and analyze interactions between geometry and molecular signals, revealing new dynamics in immune and tumor cell behaviors.

The paper uses single-cell phenomics to study DVE cell migration in mouse embryos, revealing mechanical heterogeneities and how surrounding cells facilitate collective migration.

This study identifies the ASPP2/PP1 complex as critical for maintaining pseudostratified epithelial integrity during morphogenesis by preventing apical delamination and supporting F-actin organization.

The paper identifies an autonomous Plk4 oscillator at the base of growing centrioles in Drosophila embryos that times and sets centriole biogenesis duration, independent of the cell cycle.

MOSES quantifies cellular motion phenotypes across different epithelial cell types and identifies boundary formation changes in response to external stimuli.