David Ian Stuart

This study uses cryo-ET to analyze native, active SARS-CoV-2 virions, revealing a transient open-trimer prefusion state and hidden spike flexibility with implications for vaccine design.

This paper introduces a method using femtosecond laser trenching to speed up cryo-electron tomography lamella preparation by reducing milling time and damage.

This study visualizes chromatin structure and HIV-1 nuclear trafficking in situ, showing HIV-1 cores preferentially associate with open chromatin and are excluded from compact regions.

This study visualizes the activation of semi-conservative dsRNA transcription in cystovirus Φ6 by observing capsid restructuring and transcription complex assembly using cryogenic electron microscopy.

This paper improves the expression and stability of influenza neuraminidase by grafting antigenic loops from low-expressing variants onto high-expressing scaffolds, resulting in vaccine-effective hybrid proteins.

This study produces immunogenic poliovirus virus-like particles (VLPs) in Pichia pastoris through controlled fermentation, showing improved immunogenicity compared to current IPV vaccines.

This study produces stabilized poliovirus VLPs using recombinant systems, showing equivalent or better immunogenicity than current vaccines.

The paper investigates structural plasticity in Parechovirus 2A proteins, revealing structural rearrangements that affect their oligomeric state and potential function in viral RNA replication.

The paper investigates how multivalent interactions between SARS-CoV-2 spike protein, ACE2 receptor, and patient-derived antibodies influence viral entry and antibody inhibition.

The paper identifies mutations in SARS-CoV-2 variants that confer resistance to monoclonal antibodies by analyzing genomic data and validating their impact on antibody neutralization.