John Silke

The paper identifies SCAF4-CPA and KHDRBS1/SAM68 as key regulators of RNAPII termination under CDK12 inactivation, showing termination dynamics influence elongation rate and response to inhibition.

This study shows that necroptosis in keratinocytes promotes differentiation and slows wound healing by preserving barrier integrity, suggesting it can be modulated to enhance tissue repair.

This study compares three RIPK3 kinase-dead variants to show that their scaffolding functions differ based on kinase domain conformation, not just kinase activity, and highlights implications for inhibitor development.

This study demonstrates that HOIL-1 can ubiquitinate non-proteinaceous substrates like saccharides in vitro, identifies a key residue for this activity, and produces ubiquitinated saccharides as potential tools.

The paper develops automated immunohistochemistry protocols to detect necroptotic pathway proteins in mouse and human tissues, revealing heterogeneity and disease-related changes in expression.

This study demonstrates that MLKL ubiquitylation, triggered by oligomerization, regulates necroptosis by modulating MLKL activity and turnover.