Seth Andrew Darst

This study shows desiccation-induced DNA damage in Mtb leads to mutations and drug resistance by affecting DNA repair and gene expression.

This study resolves the structural basis of Mfd loading onto stalled elongation complexes by visualizing intermediates at 3.5 Å resolution using cryo-electron microscopy.

This study shows that RNA polymerase inhibitors prevent RH-FL closure, destabilizing the trigger loop and inhibiting the nucleotide-addition cycle.

This study investigates how ppGpp regulates RNA polymerase pausing and nucleotide addition through allosteric effects and GTP competition in E. coli.

This study uses cryo-EM to visualize E. coli RNAP collisions with roadblocks and other RNAPs, revealing structural changes and mechanisms of collision resolution.

The paper predicts protein-protein interactions in Mycoplasma genitalium using a pooled-AlphaFold3 approach, improving accuracy and efficiency over traditional methods.

The paper uses structural and biochemical analysis to show that ATPase-driven translocation of σN-RI through a DNA bubble activates transcription initiation by disrupting inhibitory structures.

The paper shows that KorB, a DNA-sliding clamp, switches to a repressor role with KorA to mediate long-range gene silencing on plasmid RK2.

The paper shows RapA disrupts post-termination complexes in E. coli to prevent R-loop formation and maintain genome stability.

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

This study determines cryo-EM structures of SARS-CoV-2 NiRAN domain catalytic intermediates, revealing structural mechanisms for NMPylation and deRNAylation/capping, and explaining substrate preference for capping.

This study identifies MtbNusG as a pro-pausing transcription factor that stabilizes paused RNAP through specific structural interactions, with implications for NusG function across domains of life.