Daniel Wüstner

This study uses multimodal 3D imaging to show that lysosomes dissolve cholesterol crystals in macrophages, with cyclodextrin enhancing this process.

This study uses ion mobility and mass spectrometry imaging to identify distinct ganglioside isomers in Niemann-Pick Disease, showing therapeutic effects of gene therapy on lipid localization.

The paper uses dynamic mode decomposition to analyze live-cell imaging data and investigates KIF13B's role in ciliary transport, finding it does not affect IFT velocity or frequency in a deletion mutant.

This study shows simvastatin reduces PD-related phenotypes in iPSC-derived neurons by inhibiting RhoA, with RhoA activity varying across PD subtypes.

This study uses dynamic mode decomposition to analyze Ca2+ signaling in human astrocytes, showing cholesterol affects Ca2+ dynamics by shifting activity toward oscillatory states or suppressing it.

This paper develops a deep learning model to automate glycogen localization in TEM images of skeletal muscle, achieving high accuracy and efficiency compared to manual methods.

This study shows host-derived lipids are essential for tick cell proliferation and flavivirus output, revealing their role in regulating cellular state and viral production.

This study uses live-cell imaging and lipidomics to track LDL-derived cholesteryl esters in cells, revealing their transport and metabolism kinetics.

This study shows that the orientation of cholesterol's hydroxyl group affects membrane dynamics and intracellular transport in human astrocytes.

The paper demonstrates the SXT-100, a compact soft X-ray microscope, for correlative cryogenic biological imaging with high resolution and minimal sample damage.

This paper applies dynamic mode decomposition to analyze and predict metabolic oscillations from time-lapse imaging of cellular autofluorescence, showing its effectiveness in capturing spatiotemporal dynamics.

This paper presents novel intrinsically fluorescent cholesterol analogs with improved photophysical properties for live-cell imaging of sterol transport.

This study investigates the lipid binding properties of the yeast NCR1/NPC2 system using crystal structures and binding assays, revealing its ability to bind multiple lipids beyond sterols.

This study evaluates modified Nile Red dyes for super-resolution imaging of membrane packing in cells, finding differences in membrane structure between healthy and NPC1 disease fibroblasts.

This paper shows that ergosterol mediates natamycin aggregation in yeast plasma membranes, which underlies its antifungal activity and can be enhanced by sphingolipid inhibitors.

This study identifies a glycocalyx in yeast vacuoles and presents cryo-EM structures of NCR1 showing conformational changes that drive sterol translocation across the glycocalyx.

The paper develops a deep learning method to quantify lipophagy in yeast using fluorescence and cryo-soft X-ray microscopy data.

The paper introduces a model-free method using dynamic mode decomposition to segment fluorescence images by analyzing photobleaching kinetics, effectively separating dyes from autofluorescence and distinguishing between spectrally similar probes.

This study uses fluorescence and X-ray microscopy to show NPC2 facilitates cholesterol export from endo-lysosomes, altering membrane trafficking and organelle structure.