Miroslav Mladenov

SPIN90 selectively activates ArpC5L-containing Arp2/3 complexes to make linear actin filaments, shaping lamellipodial filament orientation and protrusion efficiency in B16 melanoma cells.

The paper reveals that human SPIN90 forms dimers to nucleate bidirectional actin filaments via Arp2/3, with structural insights showing conserved dimerisation in metazoans.

This study shows CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes, with CK-869 uniquely affecting ArpC1B and Arp3-containing complexes, impacting phagocytosis and migration in macrophages.

The paper shows that Arp2/3 complex can re-nucleate actin branches without NPF activation after debranching, suggesting a self-repair mechanism for actin networks.

This study reveals how cortactin stabilizes actin branches by interacting with activated Arp3 at the branch site, displacing other nucleation promoting factors.