Structure of a type IV CRISPR-Cas effector complex
Y. Zhou, J. P. K. Bravo, H. N. Taylor, J. Steens, R. N. Jackson, R. H. J. Staals, D. W. Taylor
Abstract
We reveal the structure of a type IV-B CRISPR effector (Csf) complex at 3.9 [A] resolution using cryo-electron microscopy. The complex resembles the type III-A CRISPR Csm effector complex, but lacks subunits for RNA processing and target DNA cleavage, and is surprisingly assembled upon heterogeneous non-CRISPR RNA. These findings provide the first glimpse into the assembly and function of enigmatic type IV CRISPR systems.
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