Heterogeneous distribution of kinesin-streptavidin complexes revealed by Mass Photometry
Jing XuiD, Nathaniel J. S. Brown, Yeonee Seol, Keir Cajal NeumaniD
Abstract
Kinesin-streptavidin complexes are widely used in microtubule-based active-matter studies. The stoichiometry of the complexes is empirically tuned but experimentally challenging to determine. Here, mass photometry measurements reveal heterogenous distributions of kinesin-streptavidin complexes. Our binding model indicates that heterogeneity arises from both the kinesin-streptavidin mixing ratio and the kinesin-biotinylation efficiency.
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