Straightforward semi-quantitative MALDI-TOF MS based screening approach for selection of recombinant protein producing E. Coli

Ivan N. Kravtsov; Andrey I. Solovyev; Eugenia A. Potemkina; Anastasya V. Kartashova; Maria A. Dmitrieva; Kseniya V. Danilova; И. Л. Тутыхина; Nikita B. Polyakov; V D Desinov; Daria A. Egorova; Gintsburg Al

doi:10.1016/j.biotno.2025.02.004

BioengineeringBiotechnology NotesJan 1, 2025

Peer-reviewed journal article

Straightforward semi-quantitative MALDI-TOF MS based screening approach for selection of recombinant protein producing E. Coli

Ivan N. Kravtsov iD, Andrey I. Solovyev iD, Eugenia A. Potemkina, Anastasya V. Kartashova, Maria A. Dmitrieva iD, Kseniya V. Danilova iD, И. Л. Тутыхина iD, Nikita B. Polyakov iD, V D Desinov, Daria A. Egorova iD, Gintsburg Al iD

Abstract

MALDI-TOF MS represents a rapid and cost-effective method for identifying proteins and microorganisms. When obtaining recombinant protein producers, differences in expression levels among transformants necessitate the conduction of a small-scale screening of expression levels. This study proposes a fast and easy method for screening clones producing recombinant proteins using MALDI-TOF MS. Various recombinant proteins were utilized to test the proposed method

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