cryo-EM

This study determines the structure of full-length human hephaestin using cryo-EM, revealing its homodimeric organization and substrate-binding site.

This study determines the cryo-EM structures of human claudin-18.2 bound to therapeutic antibodies, revealing key residues involved in antibody recognition.

This study determines cryo-EM structures of FcR bound to IgM and IgA, revealing its binding mechanism and evolutionary insights into pIg receptor recognition.

This study reports cryo-EM structures of human PAT-1 with inhibitors to elucidate their inhibition mechanisms and domain interactions.

The paper investigates how oligodendrocytes contribute to the formation of α-synuclein filaments in multiple system atrophy by comparing seeded aggregation in different cell types.

This study determines the structure of human GluD1 and elucidates its ligand-gating mechanism using cryoEM and electrophysiology.

The paper reports an extended structure of the human nuclear pore complex, revealing TREX-2 components as integral parts of the nuclear ring.

The paper presents a method for high-resolution cryoEM of nucleosomes in mammalian cell nuclear extracts without requiring thin sectioning.

This study determines the structural basis of SAMD9 autoinhibition and explains how pathogenic mutations disrupt this mechanism to cause disease.

This study identifies structural adaptability in HIV-2 Vif that enables broad antagonism of APOBEC3G through conformational selection.

This study determines the cryo-EM structure of HIV-1 capsid lattice with lenacapavir, showing how it disrupts capsid formation and function during virion maturation.

The paper determines the structure of norovirus NS3 and proposes it functions as an RNA translocase.

This study determines the structural basis of MCAM-mediated inhibition of laminin-dependent cell migration by showing MCAM and integrin compete for a shared laminin interface.

The paper presents cryo-EM structures of the Rixosome and pre-60S particles to elucidate the dynamic architecture and activation mechanism of the Rixosome for ITS2 processing.

This study determines the cryo-EM structure of TM6SF1 and shows it regulates mTORC1 signaling through cholesterol-dependent interaction with LAMTOR1, affecting TFEB activation.

This study presents cryo-EM structures of herpesvirus packaging accessory factors, revealing conserved pentameric symmetry and a critical positively charged central channel for genome packaging.

The paper uses computational and experimental methods to identify transition intermediates in the μ-opioid receptor-Gi protein complex that encode ligand efficacy.

This study presents cryo-EM structures of MBOAT2, revealing its catalytic mechanism, product retention, and ligand exchange dynamics.

This study uses cryo-EM to examine how antibodies against different PfCSP epitopes affect the structure and shedding of the circumsporozoite protein on Plasmodium falciparum sporozoites.

The paper determines the structure of HIV-1 capsid lattice bound to MxB N-terminal fragment, revealing an alpha-helix stabilizes binding at the trimer interface.

The study uses molecular dynamics simulations to show that wild-type hERG fails to conduct K+ and evolves toward an inactivated-like conformation, contrasting with the N629D mutant which maintains conductivity.

This study identifies the RY12 domain of RyR1 as an ADP sensor and reveals dantrolene's binding mechanism through cryo-EM structures.

This paper develops a multimodal framework to align protein sequences with cryo-EM density maps using joint representation learning, achieving strong retrieval performance across species and resolutions.

The paper determines how MBOAT1 remodels phosphatidylserine by solving its cryo-EM structures and combining them with lipidomics and simulations.

The paper reports the cryoEM structure of the ITG3β1-CD151 complex and shows CD151 promotes ITG3β1 surface export, explaining the epidermolysis bullosa phenotype.

The paper investigates how BIM1 selectively inhibits CLC-Ka over CLC-Kb by analyzing cryo-EM structures and showing that interactions with a conserved lysine and dynamic gating-loop behavior underlie this selectivity.

The paper designs and characterizes triazole-based peptidomimetics of the -GI conotoxin, showing they retain potency and provide structural insights into their interaction with the muscle-type nAChR.

This study designs prefusion-stabilized HSV-2 gB antigens using structure-based methods and evaluates their immunogenicity, finding no improvement in neutralizing antibody titers compared to postfusion constructs.

The paper reports the cryo-EM structure of CYP2C9, revealing a dimer-of-trimers assembly and its stabilization mechanisms.

This study determines the structures of the human NaCT protein in three states to elucidate the mechanism of sodium-substrate coupling in the DASS family.