flow cytometry

This study shows that the breast tumor microenvironment transforms eosinophils into a less active state by reducing Ly6C expression, impairing their anti-tumor function, and that IFN stimulation can partially restore this phenotype.

This study shows IL-12R signaling sustains T-bet in Treg cells, promoting type 1 specialization and enhancing suppressive function against Th1 responses.

This study shows daily HIV PrEP with TDF/FTC increases monocyte activation, enhances pro-inflammatory responses, and reprograms immune cell metabolism in HIV-negative individuals.

This study shows that yeast cells use local mitochondrial physiology to select for functional mtDNA, with purifying selection against specific mtDNA mutations under fermentative conditions.

This paper optimizes a serum-free protocol for expanding human Vγ9δ2 T cells for adoptive immunotherapy, showing high purity and viability with enhanced antitumor activity.

This study identifies immune cell populations in the developing rat pancreas and spleen, showing IUGR disrupts their composition and is influenced by tissue environment and sex.

This study tracks neutrophil dynamics during barrier challenge, revealing environment-dependent phenotypic diversity and identifying a novel CD101-low, PD-L1+ neutrophil subset with antimicrobial functions.

This study identifies a dominant population of IL-10-producing, T-bet-expressing CD4+ Tr1 cells in the small intestine that are shaped by dietary antigens rather than the microbiota.

This paper develops a high-throughput flow cytometry assay to screen for CatSper inhibitors in mouse sperm by measuring Ca2+ influx under high-K+/high-pH conditions.

The paper provides genome size and ploidy level data for 1104 non-apomictic angiosperm species from the Eastern Alps using flow cytometry on silica-dried samples.

This paper introduces post-assay photogelation to enable flow-cytometric sorting of bacterial cocultures from microfluidic droplets, achieving high-purity sorting and demonstrating its application in microbial community analysis.

The paper introduces ME-SPICy, a microfluidic platform for targeted electroporation of selected cell subpopulations using antibody-conjugated microparticles and impedance detection, achieving 5-fold enrichment of lymphocytes.

This study shows that Prevotella intestinalis induces arthritis in resistant mice by activating intestinal immune cells and promoting Th17 differentiation through IL-6.

This study identifies two compounds that reprogram tumor-associated macrophages from M2-like to M1-like antitumor phenotype using a heterospheroid-based screening platform.

The paper tracks karyotype dynamics in Macrostomum lignano using flow cytometry, showing spontaneous de novo chromosome duplications in lab cultures and proposing a method to control karyotypes.

The paper identifies PI3K-gamma signaling as a key pathway in surgery-induced MDSCs that suppresses NK cells and promotes metastases, showing that targeting this pathway can reduce postoperative metastases.

This paper introduces a flow cytometry-based assay using fluorescent virus-like particles to quantify transmembrane ligand-receptor binding, showing it can study patient-derived variants and enable single-cell analysis of multiple interactions.

This paper develops a scalable method to generate pure CD103+ cDC1 from mouse bone marrow cultures, achieving high purity and yield for immunological studies.

This study shows that repeated CBD administration in rats reduces splenic lymphocyte numbers via CB2 receptors, similar to its effects in peripheral blood.

This paper introduces a Holotomography-driven learning method for virtual staining in flow cytometry without requiring co-registration, enabling label-free single-cell analysis.

This paper introduces an automated image-based cell sorting method that enriches activated T cells from blood with high throughput and label-free morphology-based classification.

This study shows that virus-specific CD8 T cells are generated and reactivated in the bone marrow after brain viral infections, leading to changes in the bone marrow niche.

CellTrap enables long-term, single-cell analysis of immune-cancer interactions by controlling effector-to-target ratios, showing enhanced immune activity with higher ratios.

This study uses single-cell flow cytometry and statistical modeling to analyze how mouse sperm subpopulations change during capacitation in vitro, revealing nonlinear interactions between bicarbonate and calcium.

This paper introduces a Bayesian hierarchical framework to quantify both inter- and intra-experimental heterogeneity in dynamical processes using differential equations, demonstrating its robustness and flexibility.

This study uses CyTOF to profile circulating tumor cells in SCLC patients, identifying subtype-specific markers and treatment response correlations.

This study investigates the clinical impact of immunosuppressive low-density neutrophils in metastatic breast cancer, finding they correlate with faster disease progression and reduced survival.

The paper examines T-bet expression stability in virus-specific Th1 cells during chronic infection, finding that quantitative differences persist and some cells retain Tfh-like features.

This study identifies age-associated delays in immune resolution to respiratory viral infection by analyzing single-cell RNA sequencing and flow cytometry data from young and aged mice.

The paper evaluates the CytoFLEX Nano flow cytometer's performance for analyzing extracellular vesicles in complex biofluids.