flow cytometry

The paper identifies two distinct regulatory T cell subsets in colorectal cancer with opposing roles in tumor growth and prognosis.

This study identifies Aiolos as a negative regulator of antigen-inexperienced, virtual memory CD8+ T cells, showing increased TVM cell numbers and function in its absence.

This study identifies dysfunctional type 1 immunity in SSc patients through altered T cell subsets and reduced IFN-γ production.

This study identifies age-related neuroinflammatory microglia-T cell circuits in a mouse model of vascular dementia using multi-modal high-dimensional analysis.

This study shows that vitamin D reduces effector T-cell migration across the blood-brain barrier while preserving regulatory T-cell entry, potentially benefiting multiple sclerosis patients.

This study uses spectral flow cytometry to analyze age-related changes in innate and innate-like T cells, identifying age-associated phenotypic shifts in immune cell subsets.

This paper evaluates a tuberculosis vaccine regimen in mouse models, finding it highly effective in preventing reactivation and acute infection by enhancing specific immune cell populations.

The paper identifies BAP1 as a tumor suppressor in soft tissue sarcoma using an in vivo CRISPR screen and shows that PLK1 inhibition can suppress tumor growth in BAP1-deficient models.

This study optimizes in vitro expansion of Vδ1+ γδT cells from nonhuman primate PBMCs using CD3 or PHA stimulation with cytokines and phospho-vitamin C, achieving high-fold expansions.

This paper identifies human muscle reserve cells (MuRC) subpopulations using intracellular autofluorescence, finding that AFHigh cells are enriched in Pax7High cells with delayed activation and slower proliferation.

The paper models how immigration of African and North American mammals affected South American mammal diversity, finding increased overall richness but declining native diversity over time.

This paper introduces qOBM for non-destructive, label-free monitoring of T cell cultures, showing strong correlation with traditional assays and potential for improving cell therapy development.

This paper develops a mathematical model of cell state transitions in B-ALL to correlate with BCR::ABL1 status, MRD, and relapse risk using flow cytometry data.

This study identifies L-phenylalanine as a metabolic checkpoint regulating Th2 cell development, energy metabolism, and function in allergy.

This study links transdiagnostic symptom dimensions in healthy females to specific inflammatory profiles using Bayesian regression and PCA, showing associations between social withdrawal and monocyte counts, and anxious-depression with stress-induced inflammation.

This study shows that NOG-hIL-4-Tg mice support human IgG production through IL-4-dependent B cell maintenance and class switching, with varying subclass responses and tertiary lymphoid structures.

The paper presents a protocol for isolating high-quality single-cell suspensions from murine lung tissue for scRNA-seq and flow cytometry.

This study shows PD-1 blockade during T cell priming promotes an intermediate-exhausted CD8 T cell state that supports durable anti-tumor immunity after surgery and metastatic challenge, better than effector or memory-like T cells.

This study identifies pre-treatment naive CD4+ T cell abundance as a predictor of severe irAEs in patients receiving anti-PD-1/CTLA4 checkpoint inhibitor therapy for melanoma.

This paper introduces a label-free method using imaging flow cytometry to sort bacterial morphotypes from unculturable populations, achieving >90% purity for downstream analyses.

This study shows that autoimmunity and H. pylori infection together promote gastric tumorigenesis and immune suppression in mice.

This study profiles systemic immune changes in a murine OA model, finding reduced MHCII on macrophages and DCs, increased tolerogenic DCs, and shifts in T cell composition.

This study examines circulating T cell immunity to Chlamydia trachomatis in South African adolescents and young women, finding that immune response magnitude and quality correlate with infection clearance and mucosal inflammation.

This study infects rhesus macaques with a recent ONNV clinical isolate, demonstrating viremia, immune activation, and clinical disease features similar to human infection.

This study examines immune cell differences in close contacts of TB patients based on QFT status, finding minimal differences among subgroups but clear immune changes in active TB patients.

This paper shows that CD201 is a more reliable marker than SCA1 for identifying long-term reconstituting hematopoietic stem cells in mice after septic challenge.

The paper shows congenital CMV infection leads to oligoclonal expansion of cytotoxic γδ T cells with fetal-like TCR features in neonates.

This study shows that non-selective beta blockers reduce bystander CD8+ T cell activation in decompensated liver cirrhosis without affecting antigen-specific responses.