flow cytometry

Human cornea harbors tissue-resident memory T cells shaped by systemic immune activation, age and biological sex

This study identifies tissue-resident memory T cells in the human cornea and shows their abundance is influenced by age, sex, and systemic immune activation.

FlowSpot Enables Decentralized Phenotypic and Functional Cellular Immune Profiling from Dried Blood Spots

FlowSpot enables robust flow cytometric analysis of immune cells from dried blood spots, preserving immunophenotypic and functional characteristics for decentralized clinical applications.

An immune-stimulating antibody conjugate spatiotemporally targeting CD47 and TLR9 elicits macrophage-dependent tumor clearance and durable anti-cancer adaptive immunity

This paper develops an antibody conjugate targeting CD47 and TLR9 that enhances macrophage activity and induces durable anti-cancer immunity in tumor models.

A gene-agnostic FACS technique to isolate stem cells in Hydractinia symbiolongicarpus validated with cytology

The paper describes a gene-agnostic FACS method to isolate stem cells from Hydractinia symbiolongicarpus using cellular stains and validates the population with cytology.

Bacterial surface display enables lysis-independent joint host-pathogen single-cell profiling

This paper introduces a lysis-independent method for joint host-pathogen single-cell profiling using bacterial surface display, enabling analysis of both host and pathogen features in infected cells.

Randomized Spatial Barcoding for Time-Lapse Flow Cytometry

The paper introduces RASPBerry, a hydrogel-based spatial barcoding platform for time-lapse flow cytometry, enabling tracking of suspended cells with high accuracy and throughput.

Experimental Evidence for HIV-Associated Phenotypic Reprogramming of CD4⁺ T Cells: An Exploratory Immunological Study

This study investigates HIV-associated phenotypic reprogramming of CD4⁺ T cells through immunological assays, finding altered surface markers and impaired function in reprogrammed cells.

An optimized three-laser 27-color spectral flow cytometry panel for multi-organ profiling in mice

The paper develops a 27-color flow cytometry panel for 3-laser spectral cytometers to profile diverse mouse cell types across multiple tissues and identifies a new glucocorticoid-producing cell subset.

TCR-dependent and TCR-independent in-vitro T cell activation generate distinct functional, metabolic, and cytokine programs: Protein kinase C signalling augments anti-CD3+anti-CD28 responses

This study compares TCR-dependent and TCR-independent T cell activation methods, finding distinct functional, metabolic, and cytokine profiles, with PKC signaling enhancing CD3+CD28 responses.

Apoptotic bodies from patients with Sjogren's disease drive atypical memory B cell and macrophage activation and autoantibody production

The paper compares apoptotic bodies from Sjogren's disease patients and healthy donors, showing they drive B cell and macrophage activation and autoantibody production.

KIR Expression Defines a Transcriptionally Distinct CD8+ T-cell Population that Confounds Antigen-Specific T-cell Detection

This paper identifies KIR-mediated pHLA-C binding as a confounder in T-cell analysis and provides a method to distinguish it from TCR-specific binding.

Weight loss increases adipose dendritic cells, non-classical antigen presenting proteins, and cytotoxic effector cells

The paper examines how weight loss and weight cycling affect adipose tissue dendritic cells and immune responses in mice, finding increased dendritic cells and effector cells with weight loss.

Circulating immune profiling reveals impaired monocyte states and trajectories driving immunosuppression in glioblastoma

The paper identifies impaired monocyte states and trajectories in glioblastoma patients through immune profiling, revealing systemic immune reprogramming and potential therapeutic targets.

Ageing of the Upper Airway Epithelial Niche Limits Tissue-Resident T Cell Immunity

This study shows that ageing reduces tissue-resident memory T cells in the upper airway, linked to impaired epithelial-immune crosstalk and reduced TGF-β signaling.

Systemic-to-mucosal trafficking of memory B cells contributes to humoral immunity in the upper respiratory tract

The paper shows that systemic memory B cells traffic to mucosal sites in the upper respiratory tract, contributing to local immunity, and that antigen-specific B cells accumulate there only during local inflammation.

Exclusive liquid repellency isolation (ELRi) microliter scale isolation technique unmasks T-cell migration defects to CCL21 in pediatric asthma

ELRi isolates immune cells from small blood samples, revealing impaired T-cell migration to CCL21 in pediatric asthma.

IL-21-producing peripheral helper T cells associate with autoimmune bile duct injury in biliary atresia

This study identifies IL-21-producing Tph cells in tertiary lymphoid structures of biliary atresia patients and shows IL-21R blockade reduces disease severity in a mouse model.

Neutrophil myeloperoxidase as a functional biomarker for RSV severity: implications for in vitro therapeutic screening.

This study identifies neutrophil myeloperoxidase (MPO) as a biomarker for RSV severity and demonstrates its utility as a functional readout for evaluating antiviral therapies in an in vitro model.

PCIF1 loss licenses antitumour immunity via cholesterol biosynthesis

This study shows that PCIF1 loss enhances cholesterol biosynthesis, promoting antitumour immunity by increasing CD8+ T cell infiltration and improving response to immune checkpoint blockade.

Regulatory T cells establish an IL-10-IL10R immunometabolic checkpoint that limits HSL activation and lipolysis

This study identifies Tregs as an immunometabolic checkpoint that limits HSL activation and lipolysis via IL-10-IL10R signaling in adipose tissue.

Impact of chemotherapy on leukocyte stiffness -A longitudinal RT-DC study in a breast cancer patient

This study uses RT-DC to track changes in leukocyte stiffness during chemotherapy in a breast cancer patient, finding that paclitaxel alters granulo/monocyte and lymphocyte stiffness.

The anatomical compartment defines distinct immune remodeling within human visceral adipose tissue during aging

The paper examines age-related immune changes in different visceral adipose tissue depots, finding depot-specific immune remodeling linked to adjacent organ function.

Coordinated expansion of CD163⁺ monocytes and immature CD177⁺ neutrophils marks severe neurotoxicity after CD19 CAR T cell therapy

The paper identifies CD163+ monocytes and immature CD177+ neutrophils as markers of severe neurotoxicity after CD19 CAR T cell therapy through multi-omics profiling.

IgA plasma cells co-secrete monomeric and dimeric IgA

The paper shows that IgA plasma cells co-secrete monomeric and dimeric IgA, challenging the idea that distinct subsets produce only one form.

Hydrodynamic shear enables enrichment of functional tumor antigen-reactive T cells

This paper introduces a microfluidic platform using hydrodynamic shear to enrich antigen-specific T cells, achieving 35-fold enrichment from peripheral blood mononuclear cells.

High Dimensional Immune Profiling Reveals CD39 as a Correlate of Tuberculosis Disease Severity

This study uses high-dimensional immune profiling to identify CD39 as a correlate of tuberculosis disease severity and shows its potential as a therapeutic target.

CXCL13-CXCR5 Signaling in CD8⁺ T Cell Recruitment and Lymphoid Immune Organization in Clear Cell Renal Cell Carcinoma

This study identifies CXCL13-CXCR5 signaling as critical for CD8 T cell recruitment and immune organization in ccRCC, with potential as a biomarker and therapeutic target.

Longitudinal Salivary Immunophenotyping Reveals Distinct Cellular Signatures of Periodontal Disease Activity and Resolution

This paper shows that salivary immune cell profiling can track periodontal disease activity and treatment response by identifying changes in immune cell populations and inflammatory markers.

High Throughput Viral Enumeration of Aquatic Ecosystems via Flow Cytometry

This paper introduces a high throughput flow cytometry method for viral enumeration in aquatic ecosystems, improving speed and reducing contamination compared to traditional methods.

The over-activated peritoneal immune environment in endometriosis is characterised by a lack of PD-1 inhibition.

This study identifies reduced PD-1 expression in peritoneal fluid of endometriosis patients, suggesting its role in persistent inflammation and potential as a therapeutic target.