Jochen R. Guck

The paper quantifies mesoscale cellular organization in the mouse hippocampus using a new software tool, revealing cell-type-specific nuclear shape variations and positionally ordered but orientationally disordered dense regions.

This study uses optogenetic control of actin crosslinker length to show how different crosslinker lengths affect cortical mechanics and drive cell polarization or delamination.

This study uses RT-DC to track changes in leukocyte stiffness during chemotherapy in a breast cancer patient, finding that paclitaxel alters granulo/monocyte and lymphocyte stiffness.

This study uses adipose-mimetic hydrogels to show that soft mechanical cues from adipocytes enhance breast cancer cell invasion by altering ECM structure and cellular confinement.

This study shows that tumor cell viscosity affects their ability to circulate and extravasate, influencing metastasis.

This paper introduces a microfluidic method using hyperbolic channels and 3D hydrodynamic focusing to measure cell viscoelasticity, showing it can accurately characterize mechanical properties of microgel beads and HL60 cells.

QP-DC combines QPI and deformability cytometry to measure biophysical and mechanical properties of immune cells, revealing LPS-induced changes in neutrophils and heterogeneity in SLE patients.

This study uses high-throughput mechanomic screening to identify 80 genes regulating single-cell mechanics, including novel regulators, using RT-FDC technology.

This study shows cancer cells follow intrinsic self-organized programs that coordinate tumor cell state transitions during growth, revealing mechanical gradients and stereotyped patterns of heterogeneity and plasticity.

The paper introduces a method to link single-cell imaging and gene expression data through multimodal droplet barcoding, identifying genes correlated with phenotypic features.

This study uses differential dynamic microscopy to analyze cytoplasmic dynamics in Xenopus laevis egg extracts and HeLa cells, revealing subdiffusive motion and cytoskeletal influences on fluidity.

Dedifferentiated fibroblasts sequentially induce and resolve neutrophil-driven inflammation through cytokine release to facilitate axon regeneration after spinal cord injury in zebrafish.

The paper introduces optical techniques to measure molecular interaction strength and physical properties of protein condensates, showing how sequence variations and environmental factors affect these properties.

This study compares Brillouin microscopy and AFM-based indentation to assess mechanical properties of murine retinal tissues, finding divergent results and highlighting their complementary nature.

The paper investigates how cells adapt to transport oversized parasitic cargo by reprogramming mechanical forces and subcellular architecture during migration.

This paper estimates mass density from refractive index measurements using a method that incorporates biochemical composition assumptions and uncertainty analysis, validated on biological samples.

This study identifies mechanical phenotype differences between LGR5+ and LGR5- colorectal cancer cells, linking them to membrane to cortex attachment proteins and metastatic potential.

This paper introduces hyperbolic microchannels for high-throughput viscoelastic measurement of cells and soft particles, showing accurate recovery of mechanical properties under controlled stress.

The paper identifies small leucine-rich proteoglycans (SLRPs) as inhibitors of CNS regeneration by altering the structural and mechanical properties of the injury ECM.

This study shows that the nuclear-to-cytoplasmic density ratio is conserved across eukaryotes and drives cellular organization, with implications for diseases like cancer and senescence.

This study uses confocal Brillouin microscopy to assess mechanical properties during axolotl limb development and regeneration, finding a gradual increase in cartilage compressibility during these processes.

The paper shows that horizontal cells in the zebrafish retina use amoeboid-like migration to navigate crowded tissue and form the correct layer.

The paper shows that extracellular matrix topology influences collective cell migration during zebrafish optic cup formation by affecting cryptopodia formation and migration efficiency.

AIDeveloper is an open-source software for training neural networks in image classification, demonstrated on biological datasets with comparable results to conventional methods.

This study shows that acute demyelination reduces brain tissue stiffness, while inherited demyelination does not, potentially affecting remyelination in mouse models.

This study uses Brillouin microscopy to map mechanical properties of zebrafish spinal cord during development and repair, showing changes in modulus and viscosity.